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Effect of pyridostigmine on in vivo and in vitro respiratory muscle of mdx mice.
Amancio, Gabriela de Cássia Sousa; Grabe-Guimarães, Andrea; Haikel, Dridi; Moreau, Johan; Barcellos, Neila Marcia Silva; Lacampagne, Alain; Matecki, Stefan; Cazorla, Olivier.
Afiliação
  • Amancio GCS; Laboratory of Experimental Pharmacology, CiPharma, Pharmacy School, Federal University of Ouro Preto, Ouro Preto, Minas Gerais, Brazil.
  • Grabe-Guimarães A; Laboratory of Experimental Pharmacology, CiPharma, Pharmacy School, Federal University of Ouro Preto, Ouro Preto, Minas Gerais, Brazil. Electronic address: agguimar@gmail.com.
  • Haikel D; PHYMEDEXP, INSERM U1046, CNRS UMR9214, Université de Montpellier, CHRU Montpellier, Montpellier, France.
  • Moreau J; PHYMEDEXP, INSERM U1046, CNRS UMR9214, Université de Montpellier, CHRU Montpellier, Montpellier, France.
  • Barcellos NMS; Laboratory of Experimental Pharmacology, CiPharma, Pharmacy School, Federal University of Ouro Preto, Ouro Preto, Minas Gerais, Brazil.
  • Lacampagne A; PHYMEDEXP, INSERM U1046, CNRS UMR9214, Université de Montpellier, CHRU Montpellier, Montpellier, France.
  • Matecki S; PHYMEDEXP, INSERM U1046, CNRS UMR9214, Université de Montpellier, CHRU Montpellier, Montpellier, France.
  • Cazorla O; PHYMEDEXP, INSERM U1046, CNRS UMR9214, Université de Montpellier, CHRU Montpellier, Montpellier, France.
Respir Physiol Neurobiol ; 243: 107-114, 2017 09.
Article em En | MEDLINE | ID: mdl-28624507
The current work was conducted to verify the contribution of neuromuscular transmission defects at the neuromuscular junction to Duchenne Muscular Dystrophy disease progression and respiratory dysfunction. We tested pyridostigmine and pyridostigmine encapsulated in liposomes (liposomal PYR), an acetylcholinesterase inhibitor to improve muscular contraction on respiratory muscle function in mdx mice at different ages. We evaluated in vivo with the whole-body plethysmography, the ventilatory response to hypercapnia, and measured in vitro diaphragm strength in each group. Compared to C57BL10 mice, only 17 and 22 month-old mdx presented blunted ventilatory response, under normocapnia and hypercapnia. Free pyridostigmine (1mg/kg) was toxic to mdx mice, unlike liposomal PYR, which did not show any side effect, confirming that the encapsulation in liposomes is effective in reducing the toxic effects of this drug. Treatment with liposomal PYR, either acute or chronic, did not show any beneficial effect on respiratory function of this DMD experimental model. The encapsulation in liposomes is effective to abolish toxic effects of drugs.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Brometo de Piridostigmina / Transtornos Respiratórios / Músculos Respiratórios / Inibidores da Colinesterase / Distrofia Muscular de Duchenne Tipo de estudo: Etiology_studies / Prognostic_studies Limite: Animals Idioma: En Revista: Respir Physiol Neurobiol Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda
Texto completo
Adicionar na Minha BVS
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XML
PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Brometo de Piridostigmina / Transtornos Respiratórios / Músculos Respiratórios / Inibidores da Colinesterase / Distrofia Muscular de Duchenne Tipo de estudo: Etiology_studies / Prognostic_studies Limite: Animals Idioma: En Revista: Respir Physiol Neurobiol Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda