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Effect of saliva collection methods and oral hygiene on salivary biomarkers.
Justino, Allisson Benatti; Teixeira, Renata Roland; Peixoto, Leonardo Gomes; Jaramillo, Olga Lucia Bocanegra; Espindola, Foued Salmen.
Afiliação
  • Justino AB; a Institute of Genetics and Biochemistry , Federal University of Uberlandia , Uberlândia , MG , Brazil.
  • Teixeira RR; a Institute of Genetics and Biochemistry , Federal University of Uberlandia , Uberlândia , MG , Brazil.
  • Peixoto LG; a Institute of Genetics and Biochemistry , Federal University of Uberlandia , Uberlândia , MG , Brazil.
  • Jaramillo OLB; a Institute of Genetics and Biochemistry , Federal University of Uberlandia , Uberlândia , MG , Brazil.
  • Espindola FS; a Institute of Genetics and Biochemistry , Federal University of Uberlandia , Uberlândia , MG , Brazil.
Scand J Clin Lab Invest ; 77(6): 415-422, 2017 Oct.
Article em En | MEDLINE | ID: mdl-28613965
The aim of this study was to evaluate the influence of unstimulated and stimulated saliva collection methods, as well as tooth brushing, on the secretion rate of salivary total protein, nitrite, total antioxidant capacity and alpha-amylase. Saliva of 14 healthy individuals were collected with stimulation using Salivette®, Parafilm® and chewing gum and without stimulation from spit with and without fluid accumulation, before and after oral hygiene. Total protein, nitrite, total antioxidant capacity and alpha-amylase concentration (sAA) were evaluated. The collection of saliva stimulated with Parafilm® and chewing gum increased the salivary flow (1.5 ± 0.4 and 3.4 ± 0.7 mL/min, respectively) and the secretion rate of salivary total protein (1.0 ± 0.2 and 2.3 ± 0.5 mg/min, respectively). Also, chewing gum increases the salivary nitrite secretion (213 ± 58 nmol/min) and total antioxidant capacity (410 ± 47 nmol trolox eq/min). Interestingly, the unstimulated method without saliva accumulation prior to collection resulted in low sAA levels (23,531 ± 7979 pixel density). Furthermore, oral hygiene decreased salivary flow (1.3 ± 0.5 to 1.0 ± 0.4 mL/min), reduced the secretion rate of total protein (1.0 ± 0.5 to 0.6 ± 0.2 mg/min, p < .05) and increased sAA (13,159 ± 7114 to 20,075 ± 25,656 pixel density, p < .05). The type of stimulation can activate autonomous receptors responsible for the secretion and composition of saliva. Therefore, the evaluation of saliva collection methods and oral hygiene on salivary biomarkers is important for understanding and standardizing variations in salivary composition to strengthen the use of saliva as a diagnostic fluid.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Higiene Bucal / Saliva / Manejo de Espécimes / Biomarcadores Limite: Adult / Female / Humans / Male Idioma: En Revista: Scand J Clin Lab Invest Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Higiene Bucal / Saliva / Manejo de Espécimes / Biomarcadores Limite: Adult / Female / Humans / Male Idioma: En Revista: Scand J Clin Lab Invest Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido