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Mebendazole, an antiparasitic drug, inhibits drug transporters expression in preclinical model of gastric peritoneal carcinomatosis.
Celestino Pinto, Laine; de Fátima Aquino Moreira-Nunes, Caroline; Soares, Bruno Moreira; Burbano, Rommel Mário Rodriguez; de Lemos, José Alexandre Rodrigues; Montenegro, Raquel Carvalho.
Afiliação
  • Celestino Pinto L; Biological Science Institute, Federal University of Para, Belem, Pará, Brazil.
  • de Fátima Aquino Moreira-Nunes C; Laboratory of Pharmacogenomics, Nucleus of Research and Development of Medicines (NPDM), Federal University of Ceara, Fortaleza, Ceará, Brazil.
  • Soares BM; Biological Science Institute, Federal University of Para, Belem, Pará, Brazil.
  • Burbano RMR; Biological Science Institute, Federal University of Para, Belem, Pará, Brazil.
  • de Lemos JAR; Biological Science Institute, Federal University of Para, Belem, Pará, Brazil; Foundation Center for Hemotherapy and Hematology of Pará (HEMOPA), Laboratory of Genetics and Molecular Biology, Belem, Pará, Brazil.
  • Montenegro RC; Biological Science Institute, Federal University of Para, Belem, Pará, Brazil; Laboratory of Pharmacogenomics, Nucleus of Research and Development of Medicines (NPDM), Federal University of Ceara, Fortaleza, Ceará, Brazil. Electronic address: rc_montenegro@pq.cnpq.br.
Toxicol In Vitro ; 43: 87-91, 2017 Sep.
Article em En | MEDLINE | ID: mdl-28606429
The present study aimed to investigate whether MBZ down-regulates drug transporter expression (ABCB1, ABCC1, SLC47A1). mRNA expression level of ABCB1, ABCC1 and SLC47A1 was evaluated by qPCR and protein expression levels MDR-1 was performed by western blotting in malignant ascites cells (AGP-01) treated with MBZ for 24h. The mRNA expression level of ABCB1 and ABCC1 significantly decreased at a 1.0µM of MBZ compared to negative control, while SLC47A1 extremely decreased at all tested concentrations of MBZ. Protein expression levels MDR-1 significantly decreased at a 1.0µM of MBZ compared to negative control. Therefore, our results showed MBZ may play an important role in inhibiting MDR gene expression in malignant ascites cells.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias Peritoneais / Proteínas de Membrana Transportadoras / Neoplasias Gástricas / Mebendazol / Antiparasitários Limite: Humans Idioma: En Revista: Toxicol In Vitro Assunto da revista: TOXICOLOGIA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias Peritoneais / Proteínas de Membrana Transportadoras / Neoplasias Gástricas / Mebendazol / Antiparasitários Limite: Humans Idioma: En Revista: Toxicol In Vitro Assunto da revista: TOXICOLOGIA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido