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In vitro exposure of bull sperm cells to DMSA-coated maghemite nanoparticles does not affect cell functionality or structure.
Caldeira, Denise Ferreira; Paulini, Fernanda; Silva, Renata Carvalho; Azevedo, Ricardo Bentes de; Lucci, Carolina Madeira.
Afiliação
  • Caldeira DF; a Faculty of Agronomy and Veterinary Medicine , University of Brasilia , Brasilia , Brazil.
  • Paulini F; b Department of Genetics and Morphology/Department of Physiological Sciences , Institute of Biological Sciences, University of Brasilia , Brasilia , Brazil.
  • Silva RC; b Department of Genetics and Morphology/Department of Physiological Sciences , Institute of Biological Sciences, University of Brasilia , Brasilia , Brazil.
  • Azevedo RB; c Department of Genetics and Morphology , Institute of Biological Sciences, University of Brasilia , Brasilia , Brazil.
  • Lucci CM; d Department of Physiological Sciences , Institute of Biological Sciences, University of Brasilia , Brasilia , Brazil.
Int J Hyperthermia ; 34(4): 415-422, 2018 06.
Article em En | MEDLINE | ID: mdl-28605996
Magnetic nanoparticles can be used in different areas of biology. It is therefore important to know the effects of such nanomaterials on germline cells as they may traverse the blood-testis barrier. This work aimed to evaluate the response of bull sperm after exposure to a magnetic fluid containing DMSA-coated maghemite nanoparticles (MNP-DMSA) in order to determine nanotoxicity. Bull sperm was incubated with MNP-DMSA at final concentrations of 0.06, 0.03 or 0.015 mg Fe/mL. Sperm kinetics, plasma membrane integrity and acrosome reaction were evaluated over a 4 h incubation period. The sperm cells were also evaluated by transmission electron microscopy. Exposure of bull sperm to MNP-DMSA did not affect sperm kinetics or integrity. Neither ultrastructural damage of sperm cells nor uptake of nanoparticles by the spermatozoa was observed. In conclusion, MNP-DMSA does not affect sperm function or structure under the conditions tested.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Espermatozoides / Compostos Férricos / Succímero / Nanopartículas Metálicas Limite: Animals Idioma: En Revista: Int J Hyperthermia Assunto da revista: NEOPLASIAS / TERAPEUTICA Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
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XML
PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Espermatozoides / Compostos Férricos / Succímero / Nanopartículas Metálicas Limite: Animals Idioma: En Revista: Int J Hyperthermia Assunto da revista: NEOPLASIAS / TERAPEUTICA Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido