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L-Glutamine in vitro Modulates some Immunomodulatory Properties of Bone Marrow Mesenchymal Stem Cells.
Dos Santos, Guilherme Galvão; Hastreiter, Araceli Aparecida; Sartori, Talita; Borelli, Primavera; Fock, Ricardo Ambrósio.
Afiliação
  • Dos Santos GG; Laboratory of Experimental Hematology, Department of Clinical and Toxicological Analysis, Faculty of Pharmaceutical Sciences, University of São Paulo, Avenida Lineu Prestes, 580 - Bloco 17., São Paulo, SP, 05508-900, Brazil.
  • Hastreiter AA; Laboratory of Experimental Hematology, Department of Clinical and Toxicological Analysis, Faculty of Pharmaceutical Sciences, University of São Paulo, Avenida Lineu Prestes, 580 - Bloco 17., São Paulo, SP, 05508-900, Brazil.
  • Sartori T; Laboratory of Experimental Hematology, Department of Clinical and Toxicological Analysis, Faculty of Pharmaceutical Sciences, University of São Paulo, Avenida Lineu Prestes, 580 - Bloco 17., São Paulo, SP, 05508-900, Brazil.
  • Borelli P; Laboratory of Experimental Hematology, Department of Clinical and Toxicological Analysis, Faculty of Pharmaceutical Sciences, University of São Paulo, Avenida Lineu Prestes, 580 - Bloco 17., São Paulo, SP, 05508-900, Brazil.
  • Fock RA; Laboratory of Experimental Hematology, Department of Clinical and Toxicological Analysis, Faculty of Pharmaceutical Sciences, University of São Paulo, Avenida Lineu Prestes, 580 - Bloco 17., São Paulo, SP, 05508-900, Brazil. hemato@usp.br.
Stem Cell Rev Rep ; 13(4): 482-490, 2017 Aug.
Article em En | MEDLINE | ID: mdl-28593472
Glutamine (GLUT) is a nonessential amino acid that can become conditionally essential under stress conditions, being able to act in the modulation of the immune responses. Mesenchymal stem cells (MSCs) are known to their capability in the modulation of immune responses through cell-cell contact and by the secretion of soluble factors. Considering that GLUT is an immunonutrient and little is known about the influence of GLUT on the capability of MSCs to modulate immune cells, this work aims to investigate how variations in GLUT concentrations in vitro could affect some immunomodulatory properties of MSCs. In order to evaluate the effects of GLUT on MSCs immunomodulatory properties, cell proliferation rates, the expression of NFκB and STAT-3, and the production of IL-1ß, IL-6, IL-10, TGF-ß and TNF-α by MSCs were assessed. Based on our findings, GLUT at high doses (10 mM) augmented the proliferation of MSCs and modulated immune responses by decreasing levels of pro-inflammatory cytokines, such as IL-1ß and IL-6, and by increasing levels of anti-inflammatory cytokines IL-10 and TGF-ß. In addition, MSCs cultured in higher GLUT concentrations (10 mM) expressed lower levels of NF-κB and higher levels of STAT-3. Furthermore, conditioned media from MSCs cultured at higher GLUT concentrations (10 mM) reduced lymphocyte and macrophage proliferation, increased IL-10 production by both cells types, and decreased IFN-γ production by lymphocytes. Overall, this study showed that 10 mM of GLUT is able to modify immunomodulatory properties of MSCs.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células da Medula Óssea / Imunomodulação / Células-Tronco Mesenquimais / Glutamina Limite: Animals Idioma: En Revista: Stem Cell Rev Rep Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células da Medula Óssea / Imunomodulação / Células-Tronco Mesenquimais / Glutamina Limite: Animals Idioma: En Revista: Stem Cell Rev Rep Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos