Your browser doesn't support javascript.
loading
Duffy blood group system: New genotyping method and distribution in a Brazilian extra-Amazonian population.
Martins, Marina Lobato; da Silva, Adão Rogerio; Santos, Hadassa Campos; Alves, Michelle Teodoro; Schmidt, Luciana Cayres; Vertchenko, Stela Brener; Dusse, Luci Maria SantAna; Silva Malta, Maria Clara Fernandes da.
Afiliação
  • Martins ML; Fundação Centro de Hematologia e Hemoterapia de Minas Gerais - Hemominas, MG, Brazil.
  • da Silva AR; Fundação Centro de Hematologia e Hemoterapia de Minas Gerais - Hemominas, MG, Brazil; Department of Clinical and Toxicological Analysis, Faculty of Pharmacy - Universidade Federal de Minas Gerais, Brazil.
  • Santos HC; Laboratório de Genética e Cardiologia Molecular, Instituto do Coração, Faculdade de Medicina da Universidade de São Paulo, Brazil.
  • Alves MT; Fundação Centro de Hematologia e Hemoterapia de Minas Gerais - Hemominas, MG, Brazil.
  • Schmidt LC; Fundação Centro de Hematologia e Hemoterapia de Minas Gerais - Hemominas, MG, Brazil.
  • Vertchenko SB; Fundação Centro de Hematologia e Hemoterapia de Minas Gerais - Hemominas, MG, Brazil.
  • Dusse LMS; Department of Clinical and Toxicological Analysis, Faculty of Pharmacy - Universidade Federal de Minas Gerais, Brazil.
  • Silva Malta MCFD; Fundação Centro de Hematologia e Hemoterapia de Minas Gerais - Hemominas, MG, Brazil. Electronic address: maria.malta@hemominas.mg.gov.br.
Mol Cell Probes ; 35: 20-26, 2017 10.
Article em En | MEDLINE | ID: mdl-28587995
Duffy blood group system is of interest in several fields of science including transfusion medicine, immunology and malariology. Although some methods have been developed for Duffy polymorphism genotyping, not all of them have been sufficiently described and validated, and all present limitations. At the same time, the frequency of Duffy alleles and antigens in some densely populated regions of the world are still missing. In this study we present new tests for genotyping the major alleles of the Duffy blood system and describe Duffy alleles and antigens in blood donors and transfusion-dependent patients in Minas Gerais, Brazil. A simple and reproducible strategy was devised for Duffy genotyping based on real-time PCR that included SNPs rs12075 and rs2814778. No significant differences between the allele frequencies were observed comparing blood donors and patients. Among the blood donors, the phenotype Fy(a-b+) was the most common and the Fy(a-b-) phenotype, associated with populations of African descent, was remarkably less common among subjects who self-identified as black in comparison to other ethnoracial categories. However, the African ancestry estimated by molecular markers was significantly higher in individuals with the allele associated to the Duffy null phenotype. The genotyping method presented may be useful to study Duffy genotypes accurately in different contexts and populations. The results suggest a reduced risk of alloimmunization for Duffy antigens and increased susceptibility for malaria in Minas Gerais, considering the high frequency of Duffy-positive individuals.
Assuntos
Palavras-chave
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sistema do Grupo Sanguíneo Duffy / Técnicas de Genotipagem / Reação em Cadeia da Polimerase em Tempo Real Limite: Humans País/Região como assunto: America do sul / Brasil Idioma: En Revista: Mol Cell Probes Assunto da revista: BIOLOGIA MOLECULAR / BIOTECNOLOGIA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sistema do Grupo Sanguíneo Duffy / Técnicas de Genotipagem / Reação em Cadeia da Polimerase em Tempo Real Limite: Humans País/Região como assunto: America do sul / Brasil Idioma: En Revista: Mol Cell Probes Assunto da revista: BIOLOGIA MOLECULAR / BIOTECNOLOGIA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido