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Screening for biosurfactant production by 2,4,6-trinitrotoluene-transforming bacteria.
Avila-Arias, H; Avellaneda, H; Garzón, V; Rodríguez, G; Arbeli, Z; Garcia-Bonilla, E; Villegas-Plazas, M; Roldan, F.
Afiliação
  • Avila-Arias H; Unidad de Saneamiento y Biotecnología Ambiental (USBA), Departamento de Biología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, Colombia.
  • Avellaneda H; Unidad de Saneamiento y Biotecnología Ambiental (USBA), Departamento de Biología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, Colombia.
  • Garzón V; Unidad de Saneamiento y Biotecnología Ambiental (USBA), Departamento de Biología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, Colombia.
  • Rodríguez G; Unidad de Saneamiento y Biotecnología Ambiental (USBA), Departamento de Biología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, Colombia.
  • Arbeli Z; Unidad de Saneamiento y Biotecnología Ambiental (USBA), Departamento de Biología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, Colombia.
  • Garcia-Bonilla E; Unidad de Saneamiento y Biotecnología Ambiental (USBA), Departamento de Biología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, Colombia.
  • Villegas-Plazas M; Unidad de Saneamiento y Biotecnología Ambiental (USBA), Departamento de Biología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, Colombia.
  • Roldan F; Unidad de Saneamiento y Biotecnología Ambiental (USBA), Departamento de Biología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, Colombia.
J Appl Microbiol ; 123(2): 401-413, 2017 Aug.
Article em En | MEDLINE | ID: mdl-28561275
AIMS: To isolate and identify TNT-transforming cultures from explosive-contaminated soils with the ability to produce biosurfactants. METHODS AND RESULTS: Bacteria (pure and mixed cultures) were selected based on their ability to transform TNT in minimum media with TNT as the sole nitrogen source and an additional carbon source. TNT-transforming bacteria were identified by 16S rRNA gene sequencing. TNT transformation rates were significantly lower when no additional carbon or nitrogen sources were added. Surfactant production was enabled by the presence of TNT. Fourteen cultures were able to transform the explosive (>50%); of these, five showed a high transformation capacity (>90%), and six produced surfactants. CONCLUSIONS: All explosive-transforming cultures contained Proteobacteria of the genera Achromobacter, Stenotrophomonas, Pseudomonas, Sphingobium, Raoultella, Rhizobium and Methylopila. These cultures transformed TNT when an additional carbon source was added. Remarkably, Achromobacter spanius S17 and Pseudomonas veronii S94 have high TNT transformation rates and are surfactant producers. SIGNIFICANCE AND IMPACT OF THE STUDY: TNT is a highly toxic, mutagenic and carcinogenic nitroaromatic explosive; therefore, bioremediation to eliminate or mitigate its presence in the environment is essential. TNT-transforming cultures that produce surfactants are a promising method for remediation. To the best of our knowledge, this is the first report that links surfactant production and TNT transformation by bacteria.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Poluentes do Solo / Tensoativos / Bactérias / Trinitrotolueno Tipo de estudo: Diagnostic_studies / Screening_studies Idioma: En Revista: J Appl Microbiol Assunto da revista: MICROBIOLOGIA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Colômbia País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Poluentes do Solo / Tensoativos / Bactérias / Trinitrotolueno Tipo de estudo: Diagnostic_studies / Screening_studies Idioma: En Revista: J Appl Microbiol Assunto da revista: MICROBIOLOGIA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Colômbia País de publicação: Reino Unido