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Identification of karyopherins involved in the nuclear import of RNA exosome subunit Rrp6 in Saccharomyces cerevisiae.
Gonzales-Zubiate, Fernando A; Okuda, Ellen K; Da Cunha, Julia P C; Oliveira, Carla Columbano.
Afiliação
  • Gonzales-Zubiate FA; Department of Biochemistry, Institute of Chemistry, University of São Paulo, São Paulo 05508-000 SP, Brazil.
  • Okuda EK; Department of Biochemistry, Institute of Chemistry, University of São Paulo, São Paulo 05508-000 SP, Brazil.
  • Da Cunha JPC; Cell Cycle Laboratory, Center of Toxins, Immune Response and Cell Signaling-Center for Research on Toxins, Immune-response, and Cell Signaling (CeTICS), Butantan Institute, São Paulo 05503-900 SP, Brazil.
  • Oliveira CC; Department of Biochemistry, Institute of Chemistry, University of São Paulo, São Paulo 05508-000 SP, Brazil. Electronic address: ccoliv@iq.usp.br.
J Biol Chem ; 292(29): 12267-12284, 2017 07 21.
Article em En | MEDLINE | ID: mdl-28539363
The exosome is a conserved multiprotein complex essential for RNA processing and degradation. The nuclear exosome is a key factor for pre-rRNA processing through the activity of its catalytic subunits, Rrp6 and Rrp44. In Saccharomyces cerevisiae, Rrp6 is exclusively nuclear and has been shown to interact with exosome cofactors. With the aim of analyzing proteins associated with the nuclear exosome, in this work, we purified the complex with Rrp6-TAP, identified the co-purified proteins by mass spectrometry, and found karyopherins to be one of the major groups of proteins enriched in the samples. By investigating the biological importance of these protein interactions, we identified Srp1, Kap95, and Sxm1 as the most important karyopherins for Rrp6 nuclear import and the nuclear localization signals recognized by them. Based on the results shown here, we propose a model of multiple pathways for the transport of Rrp6 to the nucleus.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Saccharomyces cerevisiae / Sinais de Localização Nuclear / Carioferinas / Beta Carioferinas / Proteínas de Saccharomyces cerevisiae / Exossomos / Complexo Multienzimático de Ribonucleases do Exossomo Tipo de estudo: Diagnostic_studies / Prognostic_studies Idioma: En Revista: J Biol Chem Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
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XML
PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Saccharomyces cerevisiae / Sinais de Localização Nuclear / Carioferinas / Beta Carioferinas / Proteínas de Saccharomyces cerevisiae / Exossomos / Complexo Multienzimático de Ribonucleases do Exossomo Tipo de estudo: Diagnostic_studies / Prognostic_studies Idioma: En Revista: J Biol Chem Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos