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Evaluation of ALK gene rearrangement in central nervous system metastases of non-small-cell lung cancer using two-step RT-PCR technique.
Nicos, M; Krawczyk, P; Wojas-Krawczyk, K; Bozyk, A; Jarosz, B; Sawicki, M; Trojanowski, T; Milanowski, J.
Afiliação
  • Nicos M; Department of Pneumonology, Oncology and Allergology, Medical University of Lublin, Jaczewskiego 8, Lublin, 20-954, Poland. marcin_nicos@interia.pl.
  • Krawczyk P; Department of Pneumonology, Oncology and Allergology, Medical University of Lublin, Jaczewskiego 8, Lublin, 20-954, Poland.
  • Wojas-Krawczyk K; Department of Pneumonology, Oncology and Allergology, Medical University of Lublin, Jaczewskiego 8, Lublin, 20-954, Poland.
  • Bozyk A; Department of Pneumonology, Oncology and Allergology, Medical University of Lublin, Jaczewskiego 8, Lublin, 20-954, Poland.
  • Jarosz B; Department of Neurosurgery and Pediatric Neurosurgery, Medical University of Lublin, Lublin, 20-954, Poland.
  • Sawicki M; Department of Thoracic Surgery, Medical University of Lublin, Lublin, 20-954, Poland.
  • Trojanowski T; Department of Neurosurgery and Pediatric Neurosurgery, Medical University of Lublin, Lublin, 20-954, Poland.
  • Milanowski J; Department of Pneumonology, Oncology and Allergology, Medical University of Lublin, Jaczewskiego 8, Lublin, 20-954, Poland.
Clin Transl Oncol ; 19(12): 1447-1453, 2017 Dec.
Article em En | MEDLINE | ID: mdl-28534101
PURPOSE: RT-PCR technique has showed a promising value as pre-screening method for detection of mRNA containing abnormal ALK sequences, but its sensitivity and specificity is still discussable. Previously, we determined the incidence of ALK rearrangement in CNS metastases of NSCLC using IHC and FISH methods. MATERIALS: We evaluated ALK gene rearrangement using two-step RT-PCR method with EML4-ALK Fusion Gene Detection Kit (Entrogen, USA). The studied group included 145 patients (45 females, 100 males) with CNS metastases of NSCLC and was heterogeneous in terms of histology and smoking status. RESULTS: 21% of CNS metastases of NSCLC (30/145) showed presence of mRNA containing abnormal ALK sequences. FISH and IHC tests confirmed the presence of ALK gene rearrangement and expression of ALK abnormal protein in seven patients with positive result of RT-PCR analysis (4.8% of all patients, 20% of RT-PCR positive patients). RT-PCR method compared to FISH analysis achieved 100% of sensitivity and only 82.7% of specificity. IHC method compared to FISH method indicated 100% of sensitivity and 97.8% of specificity. In comparison to IHC, RT-PCR showed identical sensitivity with high number of false positive results. CONCLUSION: Utility of RT-PCR technique in screening of ALK abnormalities and in qualification patients for molecularly targeted therapies needs further validation.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Rearranjo Gênico / Biomarcadores Tumorais / Neoplasias do Sistema Nervoso Central / Receptores Proteína Tirosina Quinases / Carcinoma Pulmonar de Células não Pequenas / Reação em Cadeia da Polimerase Via Transcriptase Reversa Tipo de estudo: Evaluation_studies / Observational_studies / Prognostic_studies / Risk_factors_studies Limite: Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Clin Transl Oncol Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Polônia País de publicação: Itália
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Rearranjo Gênico / Biomarcadores Tumorais / Neoplasias do Sistema Nervoso Central / Receptores Proteína Tirosina Quinases / Carcinoma Pulmonar de Células não Pequenas / Reação em Cadeia da Polimerase Via Transcriptase Reversa Tipo de estudo: Evaluation_studies / Observational_studies / Prognostic_studies / Risk_factors_studies Limite: Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Clin Transl Oncol Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Polônia País de publicação: Itália