Your browser doesn't support javascript.
loading
Senecio brasiliensis impairs eclosion rate and induces apoptotic cell death in larvae of Drosophila melanogaster.
Macedo, Giulianna Echeverria; Gomes, Karen Kich; Rodrigues, Nathane Rosa; Martins, Illana Kemmerich; Wallau, Gabriel da Luz; Carvalho, Nélson Rodrigues de; Cruz, Litiele Cezar da; Costa Silva, Dennis Guilherme da; Boligon, Aline Augusti; Franco, Jeferson Luis; Posser, Thaís.
Afiliação
  • Macedo GE; Oxidative Stress and Cell Signaling Research Group, Universidade Federal do Pampa, Campus São Gabriel, 97300-000 São Gabriel, RS, Brazil.
  • Gomes KK; Oxidative Stress and Cell Signaling Research Group, Universidade Federal do Pampa, Campus São Gabriel, 97300-000 São Gabriel, RS, Brazil.
  • Rodrigues NR; Oxidative Stress and Cell Signaling Research Group, Universidade Federal do Pampa, Campus São Gabriel, 97300-000 São Gabriel, RS, Brazil.
  • Martins IK; Oxidative Stress and Cell Signaling Research Group, Universidade Federal do Pampa, Campus São Gabriel, 97300-000 São Gabriel, RS, Brazil.
  • Wallau GDL; Entomology Department, Aggeu Magalhães Research Center, Fundação Oswaldo Cruz, FIOCRUZ, 50.740-465 Recife, PE, Brazil.
  • Carvalho NR; Oxidative Stress and Cell Signaling Research Group, Universidade Federal do Pampa, Campus São Gabriel, 97300-000 São Gabriel, RS, Brazil.
  • Cruz LCD; Oxidative Stress and Cell Signaling Research Group, Universidade Federal do Pampa, Campus São Gabriel, 97300-000 São Gabriel, RS, Brazil.
  • Costa Silva DGD; Oxidative Stress and Cell Signaling Research Group, Universidade Federal do Pampa, Campus São Gabriel, 97300-000 São Gabriel, RS, Brazil.
  • Boligon AA; Phytochemical Research Laboratory, Department of Industrial Pharmacy, Federal University of Santa Maria, 97105-900 Santa Maria, RS, Brazil.
  • Franco JL; Oxidative Stress and Cell Signaling Research Group, Universidade Federal do Pampa, Campus São Gabriel, 97300-000 São Gabriel, RS, Brazil.
  • Posser T; Oxidative Stress and Cell Signaling Research Group, Universidade Federal do Pampa, Campus São Gabriel, 97300-000 São Gabriel, RS, Brazil. Electronic address: thaisposser@unipampa.edu.br.
Comp Biochem Physiol C Toxicol Pharmacol ; 198: 45-57, 2017 Aug.
Article em En | MEDLINE | ID: mdl-28529177
Senecio brasilienis (Spreng) Less., is a species native from Brazil, popularly known as "Maria mole", and known to induce hepatotoxicity due to its high content of Pyrrolizidine alkaloids. Despite its toxicity, this plant is widely used in Brazilian folk medicine. Considering the antagonizing effects described for S. brasiliensis, we describe here molecular markers involved in the toxicity of hydroalcoholic extract from leaves of S. brasiliensis (HESB) in Drosophila melanogaster. Phytochemical analysis of HESB revealed the presence of phenolic acids and flavonoids. A significant antioxidant potential against ABTS+ and DPPH radical was found in parallel. Ingestion of extract did not alter the survival and locomotor activity of adult flies. However when ingested along the larval developmental phase, the eclosion rate of flies was interrupted at higher concentration of extract. To comprehend this phenomenon several analysis were conducted in larvae. HESB stimulated activity of antioxidant enzymes SOD and GST, and increased GSH/GSSG ratio and ROS production. Additionally, HESB caused a significant decrease of cell viability. The mRNA expression of Nrf2, TrxR, CAT, Drice and Dilp6 were also significantly up-regulated. HESB caused significant decrease on the phosphorylation of MAPKs and AKT. In parallel, PARP cleavage and caspases 3/7 activity were stimulated. In addition, glucose, glycogen and triglycerides levels were decreased. Taken together our study depicts a disruption in the eclosion of D. melanogaster possibly attributed to the inhibition of kinases implied in developmental process, energetic demand and induction of apoptotic cell death process.
Assuntos
Palavras-chave
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Senécio / Extratos Vegetais / Drosophila melanogaster Limite: Animals País/Região como assunto: America do sul / Brasil Idioma: En Revista: Comp Biochem Physiol C Toxicol Pharmacol Assunto da revista: FARMACOLOGIA / TOXICOLOGIA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Senécio / Extratos Vegetais / Drosophila melanogaster Limite: Animals País/Região como assunto: America do sul / Brasil Idioma: En Revista: Comp Biochem Physiol C Toxicol Pharmacol Assunto da revista: FARMACOLOGIA / TOXICOLOGIA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos