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Efficient Production of Prebiotic Gluco-oligosaccharides in Orange Juice Using Immobilized and Co-immobilized Dextransucrase.
Tingirikari, Jagan Mohan Rao; Gomes, Wesley Faria; Rodrigues, Sueli.
Afiliação
  • Tingirikari JMR; Department of Food Science, Laboratory of Biotechnology, Federal University of Ceara, Campus do Pici, Bloco 851, Fortaleza, CE, 60440-900, Brazil. tjmr.surya@gmail.com.
  • Gomes WF; Department of Food Science, Laboratory of Biotechnology, Federal University of Ceara, Campus do Pici, Bloco 851, Fortaleza, CE, 60440-900, Brazil.
  • Rodrigues S; Department of Food Science, Laboratory of Biotechnology, Federal University of Ceara, Campus do Pici, Bloco 851, Fortaleza, CE, 60440-900, Brazil.
Appl Biochem Biotechnol ; 183(4): 1265-1281, 2017 Dec.
Article em En | MEDLINE | ID: mdl-28477145
Dextransucrase from Leuconostoc mesenteroides NRRL B-512F was subjected to immobilization and co-immobilization with dextranase from Chaetomium erraticum. Immobilization has enhanced the operational and storage stability of dextransucrase. Two hundred milligrammes (2.4 IU/mg) of alginate beads (immobilized and co-immobilized) were found to be optimum for the production of gluco-oligosaccharides (GOS) in orange juice with a high degree of polymerization. The pulp of the orange juice did not interfere in the reaction. In the batch process, co-immobilized dextransucrase (41 g/L) produced a significantly higher amount of GOS than immobilized dextransucrase (37 g/L). Alginate entrapment enhanced the thermal stability of dextransucrase for up to 3 days in orange juice at 30 °C. The production of GOS in semi-continuous process was 39 g/L in co-immobilized dextransucrase and 33 g/L in immobilized dextransucrase. Thus, immobilization technology offers a great scope in terms of reusability and efficient production of a value added functional health drink.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oligossacarídeos / Proteínas de Bactérias / Citrus sinensis / Dextranase / Enzimas Imobilizadas / Prebióticos / Sucos de Frutas e Vegetais / Glucosiltransferases / Leuconostoc Idioma: En Revista: Appl Biochem Biotechnol Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oligossacarídeos / Proteínas de Bactérias / Citrus sinensis / Dextranase / Enzimas Imobilizadas / Prebióticos / Sucos de Frutas e Vegetais / Glucosiltransferases / Leuconostoc Idioma: En Revista: Appl Biochem Biotechnol Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos