Your browser doesn't support javascript.
loading
Epithelial Ovarian Cancer Diagnosis of Second-Harmonic Generation Images: A Semiautomatic Collagen Fibers Quantification Protocol.
Zeitoune, Angel A; Luna, Johana Sj; Salas, Kynthia Sanchez; Erbes, Luciana; Cesar, Carlos L; Andrade, Liliana Ala; Carvahlo, Hernades F; Bottcher-Luiz, Fátima; Casco, Victor H; Adur, Javier.
Afiliação
  • Zeitoune AA; Biofotónica y Procesamiento de Información Biológica (ByPIB), Centro de Investigación y Transferencia de Entre Ríos (CITER), CONICET-UNER, Entre Ríos, Argentina.
  • Luna JS; Microscopy Laboratory Applied to Molecular and Cellular Studies, Engineering School, National University of Entre Ríos, Entre Ríos, Argentina.
  • Salas KS; Laboratory Applied to Non-Ionizing Radiation, Engineering School, National University of Entre Ríos, Entre Ríos, Argentina.
  • Erbes L; Laboratory Applied to Non-Ionizing Radiation, Engineering School, National University of Entre Ríos, Entre Ríos, Argentina.
  • Cesar CL; Biofotónica y Procesamiento de Información Biológica (ByPIB), Centro de Investigación y Transferencia de Entre Ríos (CITER), CONICET-UNER, Entre Ríos, Argentina.
  • Andrade LA; Microscopy Laboratory Applied to Molecular and Cellular Studies, Engineering School, National University of Entre Ríos, Entre Ríos, Argentina.
  • Carvahlo HF; National Institute of Science and Technology on Photonics Applied to Cell Biology (INFABiC), São Paulo, Brazil.
  • Bottcher-Luiz F; Department of Physics, Federal University of Ceará (UFC), Fortaleza, Brazil.
  • Casco VH; Department of Obstetrics and Gynecology, Faculty of Medical Sciences, State University of Campinas (UNICAMP), São Paulo, Brazil.
  • Adur J; National Institute of Science and Technology on Photonics Applied to Cell Biology (INFABiC), São Paulo, Brazil.
Cancer Inform ; 16: 1176935117690162, 2017.
Article em En | MEDLINE | ID: mdl-28469386
A vast number of human pathologic conditions are directly or indirectly related to tissular collagen structure remodeling. The nonlinear optical microscopy second-harmonic generation has become a powerful tool for imaging biological tissues with anisotropic hyperpolarized structures, such as collagen. During the past years, several quantification methods to analyze and evaluate these images have been developed. However, automated or semiautomated solutions are necessary to ensure objectivity and reproducibility of such analysis. This work describes automation and improvement methods for calculating the anisotropy (using fast Fourier transform analysis and the gray-level co-occurrence matrix). These were applied to analyze biopsy samples of human ovarian epithelial cancer at different stages of malignancy (mucinous, serous, mixed, and endometrial subtypes). The semiautomation procedure enabled us to design a diagnostic protocol that recognizes between healthy and pathologic tissues, as well as between different tumor types.
Palavras-chave
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Diagnostic_studies / Guideline Idioma: En Revista: Cancer Inform Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Argentina País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Diagnostic_studies / Guideline Idioma: En Revista: Cancer Inform Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Argentina País de publicação: Estados Unidos