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Low doses of IgG from atopic individuals can modulate in vitro IFN-γ production by human intra-thymic TCD4 and TCD8 cells: An IVIg comparative approach.
Sgnotto, Fábio da Ressureição; Oliveira, Marília Garcia de; Lira, Aline Aparecida de Lima; Bento-de-Souza, Luciana; Duarte, Alberto José da Silva; Victor, Jefferson Russo.
Afiliação
  • Sgnotto FDR; a Laboratory of Medical Investigation LIM-56, Division of Clinical Dermatology , Medical School, University of São Paulo , São Paulo , Brazil.
  • Oliveira MG; a Laboratory of Medical Investigation LIM-56, Division of Clinical Dermatology , Medical School, University of São Paulo , São Paulo , Brazil.
  • Lira AAL; a Laboratory of Medical Investigation LIM-56, Division of Clinical Dermatology , Medical School, University of São Paulo , São Paulo , Brazil.
  • Bento-de-Souza L; a Laboratory of Medical Investigation LIM-56, Division of Clinical Dermatology , Medical School, University of São Paulo , São Paulo , Brazil.
  • Duarte AJDS; a Laboratory of Medical Investigation LIM-56, Division of Clinical Dermatology , Medical School, University of São Paulo , São Paulo , Brazil.
  • Victor JR; b Division of Pathology , Medical School, University of São Paulo , São Paulo , Brazil.
Hum Vaccin Immunother ; 13(7): 1563-1572, 2017 07 03.
Article em En | MEDLINE | ID: mdl-28441069
The regulatory effect of allergic responses induced by IgG antibodies on human intra-thymic cells has not been reported in the literature. The aim of this study was to evaluate the possible differential effect of purified IgG from atopic and non-atopic individuals on human intra-thymic αßT cell cytokine production. Thymic tissues were obtained from 14 patients who were less than 7 d old. Additionally, blood samples were collected from atopic and non-atopic volunteers. Thymocytes and peripheral blood mononuclear cells were cultured with purified atopic or non-atopic IgG, and intracellular cytokine production was assessed. Purified IgG did not influence the frequency or viability of human intra-thymic αßT cells. Purified non-atopic IgG induced greater IFN-γ production by intra-thymic CD4+CD8+ T cells than did the mock treatment and atopic IgG. A similar effect of purified non-atopic IgG on TCD8 cells was observed compared with the mock treatment. Atopic IgG inhibited IFN-γ and TGF-ß production by intra-thymic TCD4 cells. Treatment with intravenous immunoglobulin resulted in intermediate levels of IFN-γ and TGF-ß in intra-thymic TCD4 cells compared with treatment with atopic and non-atopic IgG. Peripheral TCD4 cells from non-atopic individuals produced IFN-γ only in response to atopic IgG. This report describes novel evidence revealing that IgG from atopic individuals may influence intracellular IFN-γ production by intra-thymic αßT cells in a manner that may favor allergy development.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Linfócitos T CD4-Positivos / Interferon gama / Imunoglobulinas Intravenosas / Linfócitos T CD8-Positivos / Timócitos / Hipersensibilidade Imediata / Fatores Imunológicos Limite: Humans / Newborn Idioma: En Revista: Hum Vaccin Immunother Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Linfócitos T CD4-Positivos / Interferon gama / Imunoglobulinas Intravenosas / Linfócitos T CD8-Positivos / Timócitos / Hipersensibilidade Imediata / Fatores Imunológicos Limite: Humans / Newborn Idioma: En Revista: Hum Vaccin Immunother Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos