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Immunolocalization of steroidogenic enzymes in the vaginal mucous of Galea spixii during the estrous cycle.
Dos Santos, Amilton Cesar; Conley, Alan James; de Oliveira, Moacir Franco; Oliveira, Gleidson Benevides; Viana, Diego Carvalho; Assis Neto, Antônio Chaves de.
Afiliação
  • Dos Santos AC; Department of Surgery, School of Veterinary Medicine and Animal Science, University of São Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87 ZC 05508-270, São Paulo, Brazil.
  • Conley AJ; Population Health & Reproduction, School of Veterinary Medicine, University of California, Davis, 95616, USA.
  • de Oliveira MF; Department of Veterinary Medicine, Universidade Federal Rural do Semiárido, Mossoró, 59625-900, Brazil.
  • Oliveira GB; Department of Veterinary Medicine, Universidade Federal Rural do Semiárido, Mossoró, 59625-900, Brazil.
  • Viana DC; Department of Surgery, School of Veterinary Medicine and Animal Science, University of São Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87 ZC 05508-270, São Paulo, Brazil.
  • Assis Neto AC; Department of Surgery, School of Veterinary Medicine and Animal Science, University of São Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87 ZC 05508-270, São Paulo, Brazil. antonioassis@usp.br.
Reprod Biol Endocrinol ; 15(1): 30, 2017 Apr 24.
Article em En | MEDLINE | ID: mdl-28438170
BACKGROUND: The synthesis of sex steroids is controlled by several enzymes such as17α-hydroxylase cytochrome P450 (P450c17) catalyzing androgen synthesis and aromatase cytochrome P450 (P450arom) catalyzing estrogen synthesis, both of which must complex with the redox partner NADPH-cytochrome P450 oxidoreductase (CPR) for activity. Previous studies have identified expression of steroidogenic enzymes in vaginal tissue, suggesting local sex steroid synthesis. The current studies investigate P450c17, P450aromatase and CPR expression in vaginal mucosa of Galea spixii (Spix cavy) by immuno-histochemical and western immunoblot analyses. METHODS: Stages of estrous cyclicity were monitored by vaginal exfoliative cytology. After euthanasia, vaginal tissues were retrieved, fixed and frozen at diestrus, proestrus, estrus and metestrus. The ovaries and testis were used as positive control tissues for immunohistochemistry. RESULTS: Data from cytological study allowed identification of different estrous cycle phases. Immunohistochemical analysis showed different sites of expression of steroidogenic enzymes along with tissue response throughout different phases of the estrous cycle. However, further studies are needed to characterize the derived hormones synthesized by, and the enzymes activities associated with, vaginal tissues. CONCLUSION: Current results not only support the expression of enzymes involved in sex steroid synthesis in the wall of the vagina, they also indicate that expression changes with the stage of the cycle, both the levels and types of cells exhibiting expression. Thus, changes in proliferation of vaginal epithelial cells and the differentiation of the mucosa may be influenced by local steroid synthesis as well as circulating androgens and estrogens.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Hormônios Esteroides Gonadais / Vagina / Ciclo Estral / Proliferação de Células / Epitélio Limite: Animals Idioma: En Revista: Reprod Biol Endocrinol Assunto da revista: ENDOCRINOLOGIA / MEDICINA REPRODUTIVA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Hormônios Esteroides Gonadais / Vagina / Ciclo Estral / Proliferação de Células / Epitélio Limite: Animals Idioma: En Revista: Reprod Biol Endocrinol Assunto da revista: ENDOCRINOLOGIA / MEDICINA REPRODUTIVA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido