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The effect of G-CSF and AMD3100 on mice treated with streptozotocin: Expansion of alpha-cells and partial islet protection.
Gomez, Yonathan; Diaz-Solano, Dylana; Gledhill, Teresa; Wittig, Olga; Cardier, Jose.
Afiliação
  • Gomez Y; Unidad de Terapia Celular - Laboratorio de Patología Celular y Molecular, Centro de Medicina Experimental, Instituto Venezolano de Investigaciones Científicas (IVIC), Apartado 21827, Caracas 1020-A, Venezuela; Facultad de Ciencias, Universidad Central de Venezuela, Caracas 1080, Venezuela.
  • Diaz-Solano D; Unidad de Terapia Celular - Laboratorio de Patología Celular y Molecular, Centro de Medicina Experimental, Instituto Venezolano de Investigaciones Científicas (IVIC), Apartado 21827, Caracas 1020-A, Venezuela.
  • Gledhill T; Servicio de Anatomopatología, Hospital José María Vargas, Caracas 1080, Venezuela.
  • Wittig O; Unidad de Terapia Celular - Laboratorio de Patología Celular y Molecular, Centro de Medicina Experimental, Instituto Venezolano de Investigaciones Científicas (IVIC), Apartado 21827, Caracas 1020-A, Venezuela.
  • Cardier J; Unidad de Terapia Celular - Laboratorio de Patología Celular y Molecular, Centro de Medicina Experimental, Instituto Venezolano de Investigaciones Científicas (IVIC), Apartado 21827, Caracas 1020-A, Venezuela. Electronic address: jcardier@ivic.gob.ve.
Cytokine ; 96: 123-131, 2017 08.
Article em En | MEDLINE | ID: mdl-28399484
Administration of streptozotocin (STZ) is one of the most used experimental models of diabetes (STZ-DT). STZ induces beta-cell damage in pancreatic islets. It is known that hematopoietic stem progenitor cells (HSPCs) are mobilized from bone marrow to damaged tissues. In this work, we evaluated the effects of the hematopoietic mobilizers G-CSF (250µg/kg; for five consecutive days) and AMD3100 (5mg/kg; single s.c injection) in mice treated with STZ (175mg/kg). Mice injected with STZ showed a significant reduction in the number and area of islets and in the number of beta- and alpha-cells. Concurrently, they had hyperglycemia (blood glucose over 300mg/dl) associated with very low levels of insulin in plasma. The number and area of islets from STZ-DT mice treated with G-CSF and/or AMD3100 were similar to the controls. However, these mice had neither a reduction of hyperglycemia nor an improvement in the insulin levels. Analysis of islet cellularity showed a large reduction in beta-cells with a significant expansion of alpha-cells. These results indicate that G-CSF and AMD3100 induce partial protection of islet tissues and expansion of alpha-cells in mice treated with STZ but do not protect beta-cells from the damage induced by this compound.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fator Estimulador de Colônias de Granulócitos / Ilhotas Pancreáticas / Estreptozocina / Células Secretoras de Glucagon / Compostos Heterocíclicos Tipo de estudo: Etiology_studies / Prognostic_studies Limite: Animals Idioma: En Revista: Cytokine Assunto da revista: ALERGIA E IMUNOLOGIA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Venezuela País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fator Estimulador de Colônias de Granulócitos / Ilhotas Pancreáticas / Estreptozocina / Células Secretoras de Glucagon / Compostos Heterocíclicos Tipo de estudo: Etiology_studies / Prognostic_studies Limite: Animals Idioma: En Revista: Cytokine Assunto da revista: ALERGIA E IMUNOLOGIA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Venezuela País de publicação: Reino Unido