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Direct identification from positive blood broth culture by matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS).
Barberino, Maria Goreth; Silva, Marcio de Oliveira; Arraes, Ana Carolina Palmeiras; Correia, Luís Cláudio; Mendes, Ana Verena.
Afiliação
  • Barberino MG; Hospital São Rafael, Salvador, BA, Brazil.
  • Silva MO; Hospital São Rafael, Salvador, BA, Brazil. Electronic address: oliveiras_m@yahoo.com.br.
  • Arraes ACP; Hospital São Rafael, Salvador, BA, Brazil.
  • Correia LC; Hospital São Rafael, Salvador, BA, Brazil.
  • Mendes AV; Hospital São Rafael, Salvador, BA, Brazil.
Braz J Infect Dis ; 21(3): 339-342, 2017.
Article em En | MEDLINE | ID: mdl-28399425
Bloodstream infections (BSIs) are among the most concerning bacterial infections. They are one of the leading causes of morbidity and mortality, and occur in 30-70% of critical care patients. The prompt identification of the causative microorganism can help choosing the appropriate antimicrobial therapy that will lead to better clinical outcomes. Blood culture is one of the most relevant tests for microbiological diagnosis of bacterial infections. The introduction of the MALDI-TOF microbiological diagnosis significantly decreased the time of identifying microorganisms. However, it depends on the growth on solid culture medium. In this study, 538 bottles of positive blood cultures were evaluated to test the accuracy of an in house modified protocol. The study sample consisted of 198 Gram-negative and 350 Gram-positive bacteria. In all, 460 (83.94%) species were identified based on the direct plate findings. The protocol allowed the identification of 185/198 (93.43%) of the Gram-negative bacteria, including aerobes, anaerobes, and non-fermenters, and 275/350 (78.85%) of the Gram-positive bacteria. The proposed method has the potential to provide accurate results in comparison to the traditional method with the potential to reduce the turnaround time for the results and optimize antimicrobial therapy in BSI.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sangue / Técnicas de Tipagem Bacteriana / Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz / Bactérias Gram-Negativas / Bactérias Gram-Positivas Tipo de estudo: Diagnostic_studies / Guideline / Prognostic_studies Limite: Humans Idioma: En Revista: Braz J Infect Dis Assunto da revista: DOENCAS TRANSMISSIVEIS Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sangue / Técnicas de Tipagem Bacteriana / Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz / Bactérias Gram-Negativas / Bactérias Gram-Positivas Tipo de estudo: Diagnostic_studies / Guideline / Prognostic_studies Limite: Humans Idioma: En Revista: Braz J Infect Dis Assunto da revista: DOENCAS TRANSMISSIVEIS Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil