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Idiopathic atrophoderma of Pasini and Pierini: A case study of collagen and elastin texture by multiphoton microscopy.
Vieira-Damiani, Gislaine; Lage, Denise; Christofoletti Daldon, Patrícia Érica; Tibúrcio Alves, Caroline Romanelli; Cintra, Maria Letícia; Metze, Konradin; Adur, Javier; Pelegati, Vitor B; Carvalho, Hernandes F; Cesar, Carlos Lenz.
Afiliação
  • Vieira-Damiani G; Department of Pathology, Medical School, University of Campinas, Campinas, Brazil.
  • Lage D; Department of Dermatology, Celso Pierro Hospital, Pontifical Catholic University of Campinas, Campinas, Brazil.
  • Christofoletti Daldon PÉ; Department of Dermatology, Celso Pierro Hospital, Pontifical Catholic University of Campinas, Campinas, Brazil.
  • Tibúrcio Alves CR; Department of Dermatology, Celso Pierro Hospital, Pontifical Catholic University of Campinas, Campinas, Brazil.
  • Cintra ML; Department of Pathology, Medical School, University of Campinas, Campinas, Brazil. Electronic address: marialet@fcm.unicamp.br.
  • Metze K; Department of Pathology, Medical School, University of Campinas, Campinas, Brazil.
  • Adur J; Biophotonics Group, Department of Quantum Electronics, Institute of Physics Gleb Wataghin, University of Campinas, Campinas, Brazil.
  • Pelegati VB; Biophotonics Group, Department of Quantum Electronics, Institute of Physics Gleb Wataghin, University of Campinas, Campinas, Brazil.
  • Carvalho HF; Biophotonics Group, Department of Quantum Electronics, Institute of Physics Gleb Wataghin, University of Campinas, Campinas, Brazil; National Institute of Science and Technology on Photonics Applied to Cell Biology, University of Campinas, Campinas, Brazil; Structural and Functional Biology, Institu
  • Cesar CL; Biophotonics Group, Department of Quantum Electronics, Institute of Physics Gleb Wataghin, University of Campinas, Campinas, Brazil; National Institute of Science and Technology on Photonics Applied to Cell Biology, University of Campinas, Campinas, Brazil; Department of Physics, Federal University
J Am Acad Dermatol ; 77(5): 930-937, 2017 Nov.
Article em En | MEDLINE | ID: mdl-28389037
BACKGROUND: The diagnosis of idiopathic atrophoderma of Pasini and Pierini (IAPP) relies on typical clinical features, particularly distinctive pigmented ovular/round depressed plaques. Histologic examination often reveals no obvious changes, but patterns of collagen distribution, using multiphoton imaging and second harmonic generation can help track hidden details of tissue organization contributing to atrophy. OBJECTIVE: To identify histologic features that distinguish IAPP from unaffected skin. METHODS: Eleven patients were included for conventional analyses. Masson trichrome- and Unna-Tanzer orcein-stained sections were evaluated using automated morphometry. Hematoxylin-eosin-stained sections were analyzed by multiphoton imaging using 2-photon excited fluorescence and second harmonic generation. RESULTS: No abnormalities were found under light microscopy or by automated quantification. Multiphoton imaging revealed no difference in optical density of either collagen or elastic fibers in lesioned and unaffected skin; however, horizontal collagen fiber organization in lesion specimens increased toward the lower dermis, whereas elastic fibers featured greater disorganization within the upper dermis. LIMITATIONS: The low number of patients evaluated. CONCLUSION: The atrophic appearance of IAPP lesions reflects changes in organization, but not in collagen and elastic tissue content. Minute organizational differences that are imperceptible to the experienced pathologist and undetectable by automated analyses were revealed by multiphoton analyses, particularly second harmonic generation, in association with texture analyses.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Esclerodermia Localizada / Colágeno / Tecido Elástico Limite: Adolescent / Adult / Female / Humans / Male / Middle aged Idioma: En Revista: J Am Acad Dermatol Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Esclerodermia Localizada / Colágeno / Tecido Elástico Limite: Adolescent / Adult / Female / Humans / Male / Middle aged Idioma: En Revista: J Am Acad Dermatol Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos