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Evolution of substrate specificity in a retained enzyme driven by gene loss.
Juárez-Vázquez, Ana Lilia; Edirisinghe, Janaka N; Verduzco-Castro, Ernesto A; Michalska, Karolina; Wu, Chenggang; Noda-García, Lianet; Babnigg, Gyorgy; Endres, Michael; Medina-Ruíz, Sofía; Santoyo-Flores, Julián; Carrillo-Tripp, Mauricio; Ton-That, Hung; Joachimiak, Andrzej; Henry, Christopher S; Barona-Gómez, Francisco.
Afiliação
  • Juárez-Vázquez AL; Evolution of Metabolic Diversity Laboratory, Unidad de Genómica Avanzada (Langebio), Cinvestav-IPN, Irapuato, Mexico.
  • Edirisinghe JN; Computing, Environment and Life Sciences Directorate, Argonne National Laboratory, Lemont, United States.
  • Verduzco-Castro EA; Computation Institute, University of Chicago, Chicago.
  • Michalska K; Evolution of Metabolic Diversity Laboratory, Unidad de Genómica Avanzada (Langebio), Cinvestav-IPN, Irapuato, Mexico.
  • Wu C; Midwest Center for Structural Genomics, Biosciences Division, Argonne National Laboratory, Lemont, United States.
  • Noda-García L; Structural Biology Center, Biosciences Division, Argonne National Laboratory, Lemont, United States.
  • Babnigg G; Department of Microbiology and Molecular Genetics, University of Texas Health Science Center, Houston, United States.
  • Endres M; Evolution of Metabolic Diversity Laboratory, Unidad de Genómica Avanzada (Langebio), Cinvestav-IPN, Irapuato, Mexico.
  • Medina-Ruíz S; Midwest Center for Structural Genomics, Biosciences Division, Argonne National Laboratory, Lemont, United States.
  • Santoyo-Flores J; Midwest Center for Structural Genomics, Biosciences Division, Argonne National Laboratory, Lemont, United States.
  • Carrillo-Tripp M; Evolution of Metabolic Diversity Laboratory, Unidad de Genómica Avanzada (Langebio), Cinvestav-IPN, Irapuato, Mexico.
  • Ton-That H; Cinvestav-IPN, , Mexico.
  • Joachimiak A; Cinvestav-IPN, , Mexico.
  • Henry CS; Department of Microbiology and Molecular Genetics, University of Texas Health Science Center, Houston, United States.
  • Barona-Gómez F; Midwest Center for Structural Genomics, Biosciences Division, Argonne National Laboratory, Lemont, United States.
Elife ; 62017 03 31.
Article em En | MEDLINE | ID: mdl-28362260
The connection between gene loss and the functional adaptation of retained proteins is still poorly understood. We apply phylogenomics and metabolic modeling to detect bacterial species that are evolving by gene loss, with the finding that Actinomycetaceae genomes from human cavities are undergoing sizable reductions, including loss of L-histidine and L-tryptophan biosynthesis. We observe that the dual-substrate phosphoribosyl isomerase A or priA gene, at which these pathways converge, appears to coevolve with the occurrence of trp and his genes. Characterization of a dozen PriA homologs shows that these enzymes adapt from bifunctionality in the largest genomes, to a monofunctional, yet not necessarily specialized, inefficient form in genomes undergoing reduction. These functional changes are accomplished via mutations, which result from relaxation of purifying selection, in residues structurally mapped after sequence and X-ray structural analyses. Our results show how gene loss can drive the evolution of substrate specificity from retained enzymes.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Actinomycetaceae / Adaptação Biológica / Deleção de Genes / Aldose-Cetose Isomerases Tipo de estudo: Prognostic_studies Idioma: En Revista: Elife Ano de publicação: 2017 Tipo de documento: Article País de afiliação: México País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Actinomycetaceae / Adaptação Biológica / Deleção de Genes / Aldose-Cetose Isomerases Tipo de estudo: Prognostic_studies Idioma: En Revista: Elife Ano de publicação: 2017 Tipo de documento: Article País de afiliação: México País de publicação: Reino Unido