Your browser doesn't support javascript.
loading
Excess maternal and postnatal thyroxine alters chondrocyte numbers and the composition of the extracellular matrix of growth cartilage in rats.
Ribeiro, Lorena Gabriela Rocha; Silva, Juneo Freitas; Ocarino, Natália de Melo; de Melo, Eliane Gonçalves; Serakides, Rogéria.
Afiliação
  • Ribeiro LGR; a Núcleo de Células Tronco e Terapia Celular Animal (NCT-TCA), Escola de Veterinária , Universidade Federal de Minas Gerais , Belo Horizonte, Brazil.
  • Silva JF; b Laboratório de Endocrinologia e Metabolismo, Departamento de Fisiologia e Biofísica , Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais , Belo Horizonte, Brazil.
  • Ocarino NM; a Núcleo de Células Tronco e Terapia Celular Animal (NCT-TCA), Escola de Veterinária , Universidade Federal de Minas Gerais , Belo Horizonte, Brazil.
  • de Melo EG; c Departamento de Clínica e Cirurgia Veterinárias , Escola de Veterinária, Universidade Federal de Minas Gerais , Belo Horizonte, Brazil.
  • Serakides R; a Núcleo de Células Tronco e Terapia Celular Animal (NCT-TCA), Escola de Veterinária , Universidade Federal de Minas Gerais , Belo Horizonte, Brazil.
Connect Tissue Res ; 59(1): 73-84, 2018 01.
Article em En | MEDLINE | ID: mdl-28358226
Purpose/Aim: The aim of this study was to evaluate the effects of excess maternal and postnatal thyroxine on chondrocytes and the extracellular matrix (ECM) of growth cartilage. MATERIALS AND METHODS: We used 16 adult female Wistar rats divided into two groups: thyroxine treatment and control. From weaning to 40 days of age, offspring of the treated group (n = 8) received L-thyroxine. Plasma free T4 was measured. Histomorphometric analysis was performed on thyroids and femurs of all offspring. Alcian blue histochemical staining and real-time reverse transcription polymerase chain reaction measurements of gene expression levels of Sox9, Runx2, Aggrecan, Col I, Col II, Alkaline phosphatase, Mmp2, Mmp9, and Bmp2 were performed. Data were analyzed for statistical significance by student's t-test. RESULTS: Excess maternal and postnatal thyroxine reduced the intensity of Alcian blue staining, altered the number of chondrocytes in proliferative and hypertrophic zones in growth cartilage, and reduced the gene expression of Sox9, Mmp2, Mmp9, Col II, and Bmp2 in the growth cartilage of all offspring. Additionally, excess thyroxine altered the gene expression of Runx2, Aggrecan and Col I, and this effect was dependent on age. CONCLUSIONS: Excess thyroxine in neonates suppresses chondrocyte proliferation, stimulates chondrocyte hypertrophy and changes the ECM composition by reducing the amount of proteoglycans and glycosaminoglycans (GAGs). Prolonged exposure to excess thyroxine suppresses chondrocyte activity in general, with a severe reduction in the proteoglycan content of cartilage and the expression of gene transcripts essential for endochondral growth and characteristics of the chondrocyte phenotype.
Assuntos
Palavras-chave
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Efeitos Tardios da Exposição Pré-Natal / Tiroxina / Cartilagem / Condrócitos / Proliferação de Células / Matriz Extracelular Limite: Animals / Pregnancy Idioma: En Revista: Connect Tissue Res Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Efeitos Tardios da Exposição Pré-Natal / Tiroxina / Cartilagem / Condrócitos / Proliferação de Células / Matriz Extracelular Limite: Animals / Pregnancy Idioma: En Revista: Connect Tissue Res Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido