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Low concentration of hyaluronidase for oocyte denudation can improve fertilization rates and embryo quality.
Moura, Bernardo R L de; Gurgel, Mariana C A; Machado, Suelen Pp; Marques, Priscila A; Rolim, Juliana R; Lima, Marcelo C de; Salgueiro, Lister L.
Afiliação
  • Moura BR; Clínica Fértilis de Medicina Reprodutiva, Sorocaba, SP, Brazil.
  • Gurgel MC; Universidade Anhembi Morumbi, São Paulo, SP, Brazil.
  • Machado SP; Universidade Anhembi Morumbi, São Paulo, SP, Brazil.
  • Marques PA; Clínica Fértilis de Medicina Reprodutiva, Sorocaba, SP, Brazil.
  • Rolim JR; Clínica Fértilis de Medicina Reprodutiva, Sorocaba, SP, Brazil.
  • Lima MC; Clínica Fértilis de Medicina Reprodutiva, Sorocaba, SP, Brazil.
  • Salgueiro LL; Clínica Fértilis de Medicina Reprodutiva, Sorocaba, SP, Brazil.
JBRA Assist Reprod ; 21(1): 27-30, 2017 02 01.
Article em En | MEDLINE | ID: mdl-28333029
OBJECTIVE: Hyaluronidase enzyme is an extremely important factor for the process of oocyte denudation, but little is known about its negative effects. METHODS: This prospective randomized study analyzed the results of using different concentrations of hyaluronidase (Diluted: 8IU/mL and Normal: 80IU/mL) used for denudation of sibling-oocytes for 22 women undergoing treatment for assisted reproduction by ICSI. A total of 192 oocytes were injected, being 104 for group I (diluted) and 88 for group II (normal). We analyzed fertilization rate, cleavage, embryo quality at 48 and 72 hours and number of transferred embryos in each group. RESULTS: The diluted enzyme group showed better results in fertilization rates (92.3% vs. 80.6%), mean cleavage (4.18 ± 2.57 vs. 3.09 ± 1.90), in 48-hour embryos A and A + B (60.9% vs. 44.1% and 90.2% vs. 82.3%) and at 72 hours (45.6% vs. 36.8% and 77.1% vs 66.2%), and number of embryos selected for transfer (61.8% vs. 38.1%). The overall pregnancy rate was 59.1%. CONCLUSION: This study demonstrates that the use of 8 IU/mL of hyaluronidase, according to the following protocol, is beneficial and can be successfully used for oocyte denudation, and it is also economically advantageous to the laboratory.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oócitos / Hialuronoglucosaminidase Tipo de estudo: Clinical_trials / Guideline Limite: Female / Humans / Pregnancy Idioma: En Revista: JBRA Assist Reprod Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oócitos / Hialuronoglucosaminidase Tipo de estudo: Clinical_trials / Guideline Limite: Female / Humans / Pregnancy Idioma: En Revista: JBRA Assist Reprod Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil