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Occurrence and virulence properties of Vibrio and Salinivibrio isolates from tropical lagoons of the southern Caribbean Sea.
Fernández-Delgado, Milagro; Suárez, Paula; Giner, Sandra; Sanz, Virginia; Peña, Jessy; Sánchez, Damarys; García-Amado, M Alexandra.
Afiliação
  • Fernández-Delgado M; Centro de Biofísica y Bioquímica, Instituto Venezolano de Investigaciones Científicas (IVIC), Altos de Pipe, Venezuela.
  • Suárez P; Centro de Ingeniería de Materiales y Nanotecnología, IVIC, Altos de Pipe, Venezuela.
  • Giner S; Departamento de Biología de Organismos, Universidad Simón Bolívar (USB), Caracas, Venezuela.
  • Sanz V; Instituto de Zoología y Ecología Tropical, Universidad Central de Venezuela, Caracas, Venezuela.
  • Peña J; Centro de Ecología, IVIC, Altos de Pipe, Venezuela.
  • Sánchez D; Centro de Biofísica y Bioquímica, Instituto Venezolano de Investigaciones Científicas (IVIC), Altos de Pipe, Venezuela.
  • García-Amado MA; Centro de Biofísica y Bioquímica, Instituto Venezolano de Investigaciones Científicas (IVIC), Altos de Pipe, Venezuela.
Antonie Van Leeuwenhoek ; 110(6): 833-841, 2017 Jun.
Article em En | MEDLINE | ID: mdl-28324231
The Vibrionaceae are Gram-negative bacteria present in marine and estuarine environments worldwide, including several species known as important pathogens to humans and aquatic organisms. The aim of this research was to investigate the occurrence and virulence properties of Vibrio and Salinivibrio isolated from lagoons at Cuare Wildlife Refuge and Margarita Island in the southern Caribbean Sea. Water, plankton and oyster samples were collected during October 2011 and March 2012 and examined by specific PCR and culture methods. Vibrio genus DNA was detected in 95% of samples, while the intergenic spacer region (ISR) of Vibrio cholerae and the genes that code for the thermolabile direct haemolysin (tl) of Vibrio parahaemolyticus and the haemolysin/cytolysin (vvhA) of Vibrio vulnificus were absent or amplified in low proportions (23, 5, and 0%, respectively). Nine isolates from water and plankton were confirmed as Vibrio or Salinivibrio by phenotypic tests, 16S rRNA gene sequencing and phylogenetic analysis. All the isolates presented similar patterns of virulence factors, in which the genes ctxA (encoding for cholera toxin), tl and vvhA were lacking, whereas seven isolates displayed antibiotic resistance against ampicillin and cephalosporins. The 16S rRNA phylogenetic analysis showed the clustering of Vibrio isolates in three main clades: the plankton isolate from Cuare Wildlife Refuge formed a group with V. cholerae and Vibrio mimicus while the Margarita isolates clustered with sequences from the harveyi clade and Salinivibrio. This is the first time that Salinivibrio species are reported in tropical lagoons of the Caribbean Sea with antibiotic resistance.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Vibrio / Microbiologia da Água Limite: Animals / Humans Idioma: En Revista: Antonie Van Leeuwenhoek Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Venezuela País de publicação: Holanda
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Vibrio / Microbiologia da Água Limite: Animals / Humans Idioma: En Revista: Antonie Van Leeuwenhoek Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Venezuela País de publicação: Holanda