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Methotrexate: studies on the cellular metabolism. I. Effect on mitochondrial oxygen uptake and oxidative phosphorylation.
Yamamoto, N; Oliveira, M B; Campello, A de P; Lopes, L C; Klüppel, M L.
Afiliação
  • Yamamoto N; Departamento de Bioquímica, Universidade Federal do Paraná, Brasil.
Cell Biochem Funct ; 6(1): 61-6, 1988 Jan.
Article em En | MEDLINE | ID: mdl-2832095
Effect of methotrexate (MTX) on mitochondrial oxygen uptake, oxidative phosphorylation and on the activity of several enzymes linked to respiratory chain was studied. MTX was able to inhibit state III respiration activated by ADP and to decrease the respiratory coefficient with the substrates alpha-ketoglutarate and glutamate; these effects became pronounced when mitochondria were pre-incubated with MTX for 10 min. No effect was observed on ATPase activity of undamaged or broken mitochondria; the same was true for NADH-oxidase, NADH-dehydrogenase, NADH-cytochrome c reductase, succinate oxidase, and cytochrome c oxidase activity. The effect on the steady-state of cytochrome b, as well as, the inhibitory effect on state III of respiration with NAD+-linked substrates, offers a reasonable possibility to suggesting that the inhibition site of MTX could be in a place anterior to cytochrome b region, and not linked to respiratory chain.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fosforilação Oxidativa / Consumo de Oxigênio / Mitocôndrias Hepáticas / Metotrexato Limite: Animals Idioma: En Revista: Cell Biochem Funct Ano de publicação: 1988 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fosforilação Oxidativa / Consumo de Oxigênio / Mitocôndrias Hepáticas / Metotrexato Limite: Animals Idioma: En Revista: Cell Biochem Funct Ano de publicação: 1988 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido