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Calcium and magnesium ions modulate the oligomeric state and function of mitochondrial 2-Cys peroxiredoxins in Leishmania parasites.
Morais, Mariana A B; Giuseppe, Priscila O; Souza, Tatiana A C B; Castro, Helena; Honorato, Rodrigo V; Oliveira, Paulo S L; Netto, Luis E S; Tomas, Ana M; Murakami, Mario T.
Afiliação
  • Morais MAB; From the Biosciences National Laboratory, National Center for Research in Energy and Materials, Rua Giuseppe Maximo Scolfaro 10000, 13083-100 Campinas/SP, Brazil.
  • Giuseppe PO; From the Biosciences National Laboratory, National Center for Research in Energy and Materials, Rua Giuseppe Maximo Scolfaro 10000, 13083-100 Campinas/SP, Brazil.
  • Souza TACB; the Proteomics and Protein Engineering Laboratory, Carlos Chagas Institute, Fiocruz, Rua Professor Algacyr Munhoz Mader 2135, 81310-020 Curitiba/PR, Brazil.
  • Castro H; the i3S-Institute for Investigation and Innovation in Health, University of Porto, Rua Alfredo Allen 208, 4200-135 Porto, Portugal.
  • Honorato RV; the Institute of Molecular and Cell Biology (IBMC), University of Porto, Rua Alfredo Allen 208, 4200-135 Porto, Portugal.
  • Oliveira PSL; From the Biosciences National Laboratory, National Center for Research in Energy and Materials, Rua Giuseppe Maximo Scolfaro 10000, 13083-100 Campinas/SP, Brazil.
  • Netto LES; From the Biosciences National Laboratory, National Center for Research in Energy and Materials, Rua Giuseppe Maximo Scolfaro 10000, 13083-100 Campinas/SP, Brazil.
  • Tomas AM; the Department of Genetics and Evolutionary Biology, Institute of Biosciences, University of the State of São Paulo, Rua do Matão 14, 05508-090 São Paulo/SP, Brazil, and.
  • Murakami MT; the i3S-Institute for Investigation and Innovation in Health, University of Porto, Rua Alfredo Allen 208, 4200-135 Porto, Portugal.
J Biol Chem ; 292(17): 7023-7039, 2017 04 28.
Article em En | MEDLINE | ID: mdl-28292930
Leishmania parasites have evolved a number of strategies to cope with the harsh environmental changes during mammalian infection. One of these mechanisms involves the functional gain that allows mitochondrial 2-Cys peroxiredoxins to act as molecular chaperones when forming decamers. This function is critical for parasite infectivity in mammals, and its activation has been considered to be controlled exclusively by the enzyme redox state under physiological conditions. Herein, we have revealed that magnesium and calcium ions play a major role in modulating the ability of these enzymes to act as molecular chaperones, surpassing the redox effect. These ions are directly involved in mitochondrial metabolism and participate in a novel mechanism to stabilize the decameric form of 2-Cys peroxiredoxins in Leishmania mitochondria. Moreover, we have demonstrated that a constitutively dimeric Prx1m mutant impairs the survival of Leishmania under heat stress, supporting the central role of the chaperone function of Prx1m for Leishmania parasites during the transition from insect to mammalian hosts.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Protozoários / Cálcio / Proteínas Mitocondriais / Peroxirredoxinas / Leishmania / Magnésio Limite: Humans Idioma: En Revista: J Biol Chem Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Protozoários / Cálcio / Proteínas Mitocondriais / Peroxirredoxinas / Leishmania / Magnésio Limite: Humans Idioma: En Revista: J Biol Chem Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos