Digital expression analysis of the genes associated with salinity resistance after overexpression of a stress-responsive small GTP-binding RabG protein in peanut.

Sui, J M; Li, G; Chen, G X; Yu, M Y; Ding, S T; Wang, J S; Qiao, L X

Sui, J M; Li, G; Chen, G X; Yu, M Y; Ding, S T; Wang, J S; Qiao, L X.

Afiliação

Sui JM; College of Life Science, Qingdao Agricultural University, Key Laboratory of Qingdao Major Crop Germplasm Resource Innovation and Application, Key Lab of Plant Biotechnology in Universities of Shandong, Qingdao, Shandong, China.
Li G; College of Life Science, Qingdao Agricultural University, Key Laboratory of Qingdao Major Crop Germplasm Resource Innovation and Application, Key Lab of Plant Biotechnology in Universities of Shandong, Qingdao, Shandong, China.
Chen GX; College of Life Science, Qingdao Agricultural University, Key Laboratory of Qingdao Major Crop Germplasm Resource Innovation and Application, Key Lab of Plant Biotechnology in Universities of Shandong, Qingdao, Shandong, China.
Yu MY; College of Life Science, Qingdao Agricultural University, Key Laboratory of Qingdao Major Crop Germplasm Resource Innovation and Application, Key Lab of Plant Biotechnology in Universities of Shandong, Qingdao, Shandong, China.
Ding ST; College of Foreign Languages, Qingdao Agricultural University, Qingdao, Shandong, China.
Wang JS; College of Life Science, Qingdao Agricultural University, Key Laboratory of Qingdao Major Crop Germplasm Resource Innovation and Application, Key Lab of Plant Biotechnology in Universities of Shandong, Qingdao, Shandong, China.
Qiao LX; College of Life Science, Qingdao Agricultural University, Key Laboratory of Qingdao Major Crop Germplasm Resource Innovation and Application, Key Lab of Plant Biotechnology in Universities of Shandong, Qingdao, Shandong, China lxqiao73@163.com.

Genet Mol Res ; 16(1)2017 Mar 08.

Article em En | MEDLINE | ID: mdl-28290617

RESUMO

The Rab protein family is the largest family of the small GTP-binding proteins. Among them, the RabG genes are known to be responsive to abiotic stresses, but the molecular mechanisms of the stress responses mediated by RabG genes in plants is poorly understood. To investigate the molecular mechanism of AhRabG gene in peanut, transgenic plants overexpressing the AhRabG gene (S6) with relatively higher salinity resistance than the non-transgenic plants (S7) were obtained. Digital gene expression (DGE) sequencing was performed with the leaves of S6 and S7 plants before and after salinity-stress treatment. The AhRabG gene in peanut was found to be involved in a few pathways such as "photosynthesis", "oxidative phosphorylation", "AMPK signaling pathway", "plant hormone signal transduction", etc. A total of 298 differentially expressed genes (DEGs) were found to be upregulated or downregulated at five sampling time points based on the comparison between S6 and S7 plants. Among them, 132 DEGs were responsive to salinity stress in S6 and/or S7 after salinity-stress treatment. These 132 DEGs included genes encoding various transcription factors and proteins involved in resistance to salinity stress such as MYB, AP2, RING-H2 zinc finger proteins, late embryogenesis abundant (LEA) proteins, dehydration-responsive protein RD22, peroxidases, CBL-interacting protein kinases, calcium-binding proteins, and others. The information from this study will be useful for further studies on elucidating the mechanism of salinity resistance conferred by RabG genein peanut.

Assuntos

Arachis/genética; Proteínas de Plantas/biossíntese; Plantas Tolerantes a Sal/genética; Proteínas rab de Ligação ao GTP/biossíntese; Adaptação Fisiológica/genética; Arachis/metabolismo; Proteínas de Ligação ao Cálcio/genética; Secas; Folhas de Planta/genética; Folhas de Planta/metabolismo; Proteínas de Plantas/genética; Plantas Geneticamente Modificadas; Salinidade; Plantas Tolerantes a Sal/metabolismo; Transdução de Sinais; Estresse Fisiológico/genética; Fatores de Transcrição/biossíntese; Fatores de Transcrição/genética; Proteínas rab de Ligação ao GTP/genética; Proteínas rab de Ligação ao GTP/metabolismo

Texto completo

Adicionar na Minha BVS

Imprimir

XML

PubMed Links

Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Arachis / Proteínas de Plantas / Proteínas rab de Ligação ao GTP / Plantas Tolerantes a Sal Tipo de estudo: Risk_factors_studies Idioma: En Revista: Genet Mol Res Assunto da revista: BIOLOGIA MOLECULAR / GENETICA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: China País de publicação: Brasil

Texto completo

Adicionar na Minha BVS

Imprimir

XML

PubMed Links

Buscar no Google