Your browser doesn't support javascript.
loading
Characterization of MicroRNA Expression Profiles and Identification of Potential Biomarkers in Leprosy.
Jorge, Karina T O S; Souza, Renan P; Assis, Marieta T A; Araújo, Marcelo G; Locati, Massimo; Jesus, Amélia M R; Dias Baptista, Ida M F; Lima, Cristiano X; Teixeira, Antônio L; Teixeira, Mauro M; Soriani, Frederico M.
Afiliação
  • Jorge KTOS; Departamento de Biologia Geral, Universidade Federal de Minas Gerais, Minas Gerais, Brazil.
  • Souza RP; Departamento de Biologia Geral, Universidade Federal de Minas Gerais, Minas Gerais, Brazil.
  • Assis MTA; Departamento de Biologia Geral, Universidade Federal de Minas Gerais, Minas Gerais, Brazil.
  • Araújo MG; Departamento de Clínica Médica, Faculdade de Medicina, Universidade Federal de Minas Gerais, Minas Gerais, Brazil.
  • Locati M; Humanitas Clinical and Research Center, University of Milan, Milan, Italy.
  • Jesus AMR; Departamento de Medicina Interna e Patologia, Universidade Federal de Sergipe, Sergipe, Brazil.
  • Dias Baptista IMF; Instituto Lauro de Souza Lima, São Paulo, Brazil.
  • Lima CX; Departamento de Cirurgia, Faculdade de Medicina, Universidade Federal de Minas Gerais, Minas Gerais, Brazil.
  • Teixeira AL; Departamento de Clínica Médica, Faculdade de Medicina, Universidade Federal de Minas Gerais, Minas Gerais, Brazil.
  • Teixeira MM; Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Minas Gerais, Brazil.
  • Soriani FM; Departamento de Biologia Geral, Universidade Federal de Minas Gerais, Minas Gerais, Brazil fredsori@icb.ufmg.br.
J Clin Microbiol ; 55(5): 1516-1525, 2017 05.
Article em En | MEDLINE | ID: mdl-28275081
Leprosy is an important cause of disability in the developing world. Early diagnosis is essential to allow for cure and to interrupt transmission of this infection. MicroRNAs (miRNAs) are important factors for host-pathogen interaction and they have been identified as biomarkers for various infectious diseases. The expression profile of 377 microRNAs were analyzed by TaqMan low-density array (TLDA) in skin lesions of tuberculoid and lepromatous leprosy patients as well as skin specimens from healthy controls. In a second step, 16 microRNAs were selected for validation experiments with reverse transcription-quantitative PCR (qRT-PCR) in skin samples from new individuals. Principal-component analysis followed by logistic regression model and receiver operating characteristic (ROC) curve analyses were performed to evaluate the diagnostic potential of selected miRNAs. Four patterns of differential expression were identified in the TLDA experiment, suggesting a diagnostic potential of miRNAs in leprosy. After validation experiments, a combination of four miRNAs (miR-101, miR-196b, miR-27b, and miR-29c) was revealed as able to discriminate between healthy control and leprosy patients with 80% sensitivity and 91% specificity. This set of miRNAs was also able to discriminate between lepromatous and tuberculoid patients with a sensitivity of 83% and 80% specificity. In this work, it was possible to identify a set of miRNAs with good diagnostic potential for leprosy.
Assuntos
Palavras-chave
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Marcadores Genéticos / MicroRNAs / Hanseníase / Mycobacterium leprae Tipo de estudo: Diagnostic_studies / Prognostic_studies / Screening_studies Limite: Adult / Humans / Middle aged Idioma: En Revista: J Clin Microbiol Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Marcadores Genéticos / MicroRNAs / Hanseníase / Mycobacterium leprae Tipo de estudo: Diagnostic_studies / Prognostic_studies / Screening_studies Limite: Adult / Humans / Middle aged Idioma: En Revista: J Clin Microbiol Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos