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Different genotypes of Trypanosoma cruzi produce distinctive placental environment genetic response in chronic experimental infection.
Juiz, Natalia Anahí; Solana, María Elisa; Acevedo, Gonzalo Raúl; Benatar, Alejandro Francisco; Ramirez, Juan Carlos; da Costa, Priscilla Almeida; Macedo, Andrea Mara; Longhi, Silvia Andrea; Schijman, Alejandro G.
Afiliação
  • Juiz NA; Grupo de Biología Molecular de la Enfermedad de Chagas (LaBMECh), Instituto de Investigaciones en Ingeniería Genética y Biología Molecular ''Dr. Héctor N. Torres" (INGEBI-CONICET), Buenos Aires, Argentina.
  • Solana ME; Departamento de Microbiología, Parasitología e Inmunología, Instituto de Investigaciones en Microbiología y Parasitología Médica (IMPaM), Facultad de Medicina, Universidad de Buenos Aires (UBA), Buenos Aires, Argentina.
  • Acevedo GR; Grupo de Biología Molecular de la Enfermedad de Chagas (LaBMECh), Instituto de Investigaciones en Ingeniería Genética y Biología Molecular ''Dr. Héctor N. Torres" (INGEBI-CONICET), Buenos Aires, Argentina.
  • Benatar AF; Grupo de Biología Molecular de la Enfermedad de Chagas (LaBMECh), Instituto de Investigaciones en Ingeniería Genética y Biología Molecular ''Dr. Héctor N. Torres" (INGEBI-CONICET), Buenos Aires, Argentina.
  • Ramirez JC; Grupo de Biología Molecular de la Enfermedad de Chagas (LaBMECh), Instituto de Investigaciones en Ingeniería Genética y Biología Molecular ''Dr. Héctor N. Torres" (INGEBI-CONICET), Buenos Aires, Argentina.
  • da Costa PA; Departamento de Bioquímica e Inmunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
  • Macedo AM; Departamento de Bioquímica e Inmunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
  • Longhi SA; Grupo de Biología Molecular de la Enfermedad de Chagas (LaBMECh), Instituto de Investigaciones en Ingeniería Genética y Biología Molecular ''Dr. Héctor N. Torres" (INGEBI-CONICET), Buenos Aires, Argentina.
  • Schijman AG; Grupo de Biología Molecular de la Enfermedad de Chagas (LaBMECh), Instituto de Investigaciones en Ingeniería Genética y Biología Molecular ''Dr. Héctor N. Torres" (INGEBI-CONICET), Buenos Aires, Argentina.
PLoS Negl Trop Dis ; 11(3): e0005436, 2017 03.
Article em En | MEDLINE | ID: mdl-28273076
Congenital infection of Trypanosoma cruzi allows transmission of this parasite through generations. Despite the problematic that this entails, little is known about the placenta environment genetic response produced against infection. We performed functional genomics by microarray analysis in C57Bl/6J mice comparing placentas from uninfected animals and from animals infected with two different T. cruzi strains: K98, a clone of the non-lethal myotropic CA-I strain (TcI), and VD (TcVI), isolated from a human case of congenital infection. Analysis of networks by GeneMANIA of differentially expressed genes showed that "Secretory Granule" was a pathway down-regulated in both infected groups, whereas "Innate Immune Response" and "Response to Interferon-gamma" were pathways up-regulated in VD infection but not in K98. Applying another approach, the GSEA algorithm that detects small changes in predetermined gene sets, we found that metabolic processes, transcription and macromolecular transport were down-regulated in infected placentas environment and some pathways related to cascade signaling had opposite regulation: over-represented in VD and down-regulated in K98 group. We also have found a stronger tropism to the placental organ by VD strain, by detection of parasite DNA and RNA, suggesting living parasites. Our study is the first one to describe in a murine model the genetic response of placental environment to T. cruzi infection and suggests the development of a strong immune response, parasite genotype-dependent, to the detriment of cellular metabolism, which may contribute to control infection preventing the risk of congenital transmission.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Placenta / Complicações Infecciosas na Gravidez / Trypanosoma cruzi / Doença de Chagas / Genótipo Limite: Animals / Pregnancy Idioma: En Revista: PLoS Negl Trop Dis Assunto da revista: MEDICINA TROPICAL Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Argentina País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Placenta / Complicações Infecciosas na Gravidez / Trypanosoma cruzi / Doença de Chagas / Genótipo Limite: Animals / Pregnancy Idioma: En Revista: PLoS Negl Trop Dis Assunto da revista: MEDICINA TROPICAL Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Argentina País de publicação: Estados Unidos