Glyceroneogenesis in the hepatopancreas of the crab Neohelice granulata: Diet, starvation and season effects.

Sarapio, E; Santos, J T; Model, J F A; De Fraga, L S; Vinagre, A S; Martins, T L; Da Silva, R S M; Trapp, M

Sarapio, E; Santos, J T; Model, J F A; De Fraga, L S; Vinagre, A S; Martins, T L; Da Silva, R S M; Trapp, M.

Afiliação

Sarapio E; Department of Physiology, Institute of Health Basic Sciences, Federal University of Rio Grande do Sul, Rua Sarmento Leite, 500, 90050-170 Porto Alegre, RS, Brazil.
Santos JT; Department of Physiology, Institute of Health Basic Sciences, Federal University of Rio Grande do Sul, Rua Sarmento Leite, 500, 90050-170 Porto Alegre, RS, Brazil.
Model JFA; Department of Physiology, Institute of Health Basic Sciences, Federal University of Rio Grande do Sul, Rua Sarmento Leite, 500, 90050-170 Porto Alegre, RS, Brazil.
De Fraga LS; Department of Physiology, Institute of Health Basic Sciences, Federal University of Rio Grande do Sul, Rua Sarmento Leite, 500, 90050-170 Porto Alegre, RS, Brazil.
Vinagre AS; Department of Physiology, Institute of Health Basic Sciences, Federal University of Rio Grande do Sul, Rua Sarmento Leite, 500, 90050-170 Porto Alegre, RS, Brazil.
Martins TL; Department of Physiology, Institute of Health Basic Sciences, Federal University of Rio Grande do Sul, Rua Sarmento Leite, 500, 90050-170 Porto Alegre, RS, Brazil.
Da Silva RSM; Department of Physiology, Institute of Health Basic Sciences, Federal University of Rio Grande do Sul, Rua Sarmento Leite, 500, 90050-170 Porto Alegre, RS, Brazil.
Trapp M; Department of Physiology, Institute of Health Basic Sciences, Federal University of Rio Grande do Sul, Rua Sarmento Leite, 500, 90050-170 Porto Alegre, RS, Brazil. Electronic address: mtrapp@ufrgs.br.

Comp Biochem Physiol B Biochem Mol Biol ; 211: 1-7, 2017 Sep.

Article em En | MEDLINE | ID: mdl-28254380

RESUMO

We determined the activity of glyceroneogenesis from [2-14C]-pyruvate, the phosphoenolpyruvate carboxykinase activity, [2-14C]-pyruvate oxidation and total lipid levels in the hepatopancreas of the crab Neohelice granulata fed with a carbohydrate-rich (HC) diet or a high-protein (HP) diet and then subjected to 5weeks of starvation, in summer and winter, to determine whether the seasonal adjustments of lipid metabolism to food scarcity are modulated by the composition of the diet previously given to the crabs. The results demonstrated that glyceroneogenesis is an active pathway in N. granulata hepatopancreas, and is regulated by seasonal variations, diet composition and starvation. This study showed that in summer the increase in the hepatopancreas glyceroneogenesis activity is among the strategies used by N. granulata fed an HP diet, to maintain the triglyceride/fatty acid cycle during starvation, a normal condition in the biological cycle of this crab. However, the administration of an HC diet reduced the glyceroneogenesis capacity in response to starvation in summer. In winter, the decrease in the glyceroneogenesis capacity in both fed (HP and HC diets) and starved crabs seems to be a strategy to reduce energy consumption and/or requirement. In contrast to the summer results, the incorporation of [2-14C]-pyruvate into 14CO2 was markedly higher in both diet (HC and HP) groups and in starved crabs during the winter. Four decades after the first study describing the glyceroneogenesis pathway in rat white adipose tissue, this pathway is evidenced for the first time in a crustacean.

Assuntos

Braquiúros/metabolismo; Dieta; Gluconeogênese/fisiologia; Glucose/metabolismo; Glicerol/metabolismo; Hepatopâncreas/metabolismo; Inanição; Animais; Carboidratos da Dieta/metabolismo; Proteínas Alimentares/metabolismo; Ácidos Graxos/metabolismo; Estações do Ano

Palavras-chave

Carbohydrate-rich diet; Crab; Glyceroneogenesis; High-protein diet; Starvation; Summer; Winter

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Inanição / Braquiúros / Hepatopâncreas / Dieta / Gluconeogênese / Glucose / Glicerol Limite: Animals Idioma: En Revista: Comp Biochem Physiol B Biochem Mol Biol Assunto da revista: BIOLOGIA MOLECULAR / BIOQUIMICA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido

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