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-Genomic data mining of the marine actinobacteria Streptomyces sp. H-KF8 unveils insights into multi-stress related genes and metabolic pathways involved in antimicrobial synthesis.
Undabarrena, Agustina; Ugalde, Juan A; Seeger, Michael; Cámara, Beatriz.
Afiliação
  • Undabarrena A; Departmento de Química & Centro de Biotecnología, Universidad Técnica Federico Santa María , Valparaiso , Chile.
  • Ugalde JA; Centro de Genética y Genómica, Facultad de Medicina Clinica Alemana, Universidad del Desarrollo , Santiago , Chile.
  • Seeger M; Departmento de Química & Centro de Biotecnología, Universidad Técnica Federico Santa María , Valparaiso , Chile.
  • Cámara B; Departmento de Química & Centro de Biotecnología, Universidad Técnica Federico Santa María , Valparaiso , Chile.
PeerJ ; 5: e2912, 2017.
Article em En | MEDLINE | ID: mdl-28229018
Streptomyces sp. H-KF8 is an actinobacterial strain isolated from marine sediments of a Chilean Patagonian fjord. Morphological characterization together with antibacterial activity was assessed in various culture media, revealing a carbon-source dependent activity mainly against Gram-positive bacteria (S. aureus and L. monocytogenes). Genome mining of this antibacterial-producing bacterium revealed the presence of 26 biosynthetic gene clusters (BGCs) for secondary metabolites, where among them, 81% have low similarities with known BGCs. In addition, a genomic search in Streptomyces sp. H-KF8 unveiled the presence of a wide variety of genetic determinants related to heavy metal resistance (49 genes), oxidative stress (69 genes) and antibiotic resistance (97 genes). This study revealed that the marine-derived Streptomyces sp. H-KF8 bacterium has the capability to tolerate a diverse set of heavy metals such as copper, cobalt, mercury, chromate and nickel; as well as the highly toxic tellurite, a feature first time described for Streptomyces. In addition, Streptomyces sp. H-KF8 possesses a major resistance towards oxidative stress, in comparison to the soil reference strain Streptomyces violaceoruber A3(2). Moreover, Streptomyces sp. H-KF8 showed resistance to 88% of the antibiotics tested, indicating overall, a strong response to several abiotic stressors. The combination of these biological traits confirms the metabolic versatility of Streptomyces sp. H-KF8, a genetically well-prepared microorganism with the ability to confront the dynamics of the fjord-unique marine environment.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: PeerJ Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Chile País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: PeerJ Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Chile País de publicação: Estados Unidos