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Regulation of semaphorin 4D expression and cell proliferation of ovarian cancer by ERalpha and ERbeta.
Liu, Y; Hou, Y; Ma, L; Sun, C; Pan, J; Yang, Y; Zhou, H; Zhang, J.
Afiliação
  • Liu Y; Department of Gynecology, the Second Affiliated Hospital, Kunming Medical University, Kunming, Yunnan, China.
  • Hou Y; Department of Gynecology, the Second Affiliated Hospital, Kunming Medical University, Kunming, Yunnan, China.
  • Ma L; Department of Reproduction and Genetics, the Second Affiliated Hospital, Kunming Medical University, Kunming, Yunnan, China.
  • Sun C; Department of Gynecology, the Second Affiliated Hospital, Kunming Medical University, Kunming, Yunnan, China.
  • Pan J; Department of Gynecology, the Second Affiliated Hospital, Kunming Medical University, Kunming, Yunnan, China.
  • Yang Y; Department of Gynecology, the Second Affiliated Hospital, Kunming Medical University, Kunming, Yunnan, China.
  • Zhou H; Department of Gynecology, the Second Affiliated Hospital, Kunming Medical University, Kunming, Yunnan, China.
  • Zhang J; Department of General Surgery, the Second Affiliated Hospital, Kunming Medical University, Kunming, Yunnan, China.
Braz J Med Biol Res ; 50(3): e6057, 2017 Feb 20.
Article em En | MEDLINE | ID: mdl-28225892
Ovarian cancer is one of the most common malignancies in women. Semaphorin 4D (sema 4D) is involved in the progress of multiple cancers. In the presence of estrogen-like ligands, estrogen receptors (ERα and ERß) participate in the progress of breast and ovarian cancers by transcriptional regulation. The aim of the study was to investigate the role of sema 4D and elucidate the regulatory pattern of ERα and ERß on sema 4D expression in ovarian cancers. Sema 4D levels were up-regulated in ovarian cancer SKOV-3 cells. Patients with malignant ovarian cancers had significantly higher sema 4D levels than controls, suggesting an oncogene role of sema 4D in ovarian cancer. ERα expressions were up-regulated in SKOV-3 cells compared with normal ovarian IOSE80 epithelial cells. Conversely, down-regulation of ERß was observed in SKOV-3 cells. Forced over-expression of ERα and ERß in SKOV-3 cells was manipulated to establish ERα+ and ERß+ SKOV-3 cell lines. Incubation of ERα+ SKOV-3 cells with ERs agonist 17ß-estradiol (E2) significantly enhanced sema 4D expression and rate of cell proliferation. Incubated with E2, ERß+ SKOV-3 cells showed lower sema 4D expression and cell proliferation. Blocking ERα and ERß activities with ICI182-780 inhibitor, sema 4D expressions and cell proliferation of ERα+ and ERß+ SKOV-3 cells were recovered to control levels. Taken together, the data showed that sema 4D expression was positively correlated with the progress of ovarian cancer. ERα positively regulated sema 4D expression and accelerated cell proliferation. ERß negatively regulated sema 4D expression and inhibited cell multiplication.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias Ovarianas / Semaforinas / Receptor alfa de Estrogênio / Receptor beta de Estrogênio Limite: Female / Humans Idioma: En Revista: Braz J Med Biol Res Ano de publicação: 2017 Tipo de documento: Article País de afiliação: China País de publicação: Brasil
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias Ovarianas / Semaforinas / Receptor alfa de Estrogênio / Receptor beta de Estrogênio Limite: Female / Humans Idioma: En Revista: Braz J Med Biol Res Ano de publicação: 2017 Tipo de documento: Article País de afiliação: China País de publicação: Brasil