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Cytotoxic analysis and chemical characterization of fractions of the hydroalcoholic extract of the Euterpe oleracea Mart. seed in the MCF-7 cell line.
Freitas, Dayanne da S; Morgado-Díaz, José A; Gehren, Adriana S; Vidal, Flávia C B; Fernandes, Raquel Maria T; Romão, Wanderson; Tose, Lilian V; Frazão, Fabiola N S; Costa, Maria Célia P; Silva, Dulcelena F; Nascimento, Maria do Desterro S B.
Afiliação
  • Freitas DDS; Post-Graduation Program in Adult and Children Health, Research Center of CCBS, Federal University of Maranhão, São Luís, Maranhão, Brazil.
  • Morgado-Díaz JA; Cellular Biology Program, Brazilian National Cancer Institute (INCA), Rio de Janeiro, RJ, Brazil.
  • Gehren AS; Cellular Biology Program, Brazilian National Cancer Institute (INCA), Rio de Janeiro, RJ, Brazil.
  • Vidal FCB; Post-Graduation Program in Adult and Children Health, Research Center of CCBS, Federal University of Maranhão, São Luís, Maranhão, Brazil.
  • Fernandes RMT; Morphology Department, Federal University of Maranhão, São Luís, Maranhão, Brazil.
  • Romão W; Center of Exact and Natural Sciences, Stadual University of Maranhão, São Luís, Maranhão, Brazil.
  • Tose LV; Petroleômica and Forensic Laboratory, Chemistry Department, Federal University Espírito Santo, Vitória, Espírito Santo, Brazil.
  • Frazão FNS; Federal Institute of Education, Science and Technology of Espírito Santo, Vila Velha, Espírito Santo, Brazil.
  • Costa MCP; Petroleômica and Forensic Laboratory, Chemistry Department, Federal University Espírito Santo, Vitória, Espírito Santo, Brazil.
  • Silva DF; Scholarship Scientific Initiation PIBIC-CNPq/UFMA, Federal University of Maranhão, São Luís, Maranhão, Brazil.
  • Nascimento MDDSB; Center of Exact and Natural Sciences, Stadual University of Maranhão, São Luís, Maranhão, Brazil.
J Pharm Pharmacol ; 69(6): 714-721, 2017 Jun.
Article em En | MEDLINE | ID: mdl-28211563
OBJECTIVES: To analyse the antineoplastic activity of fractions derived from the hydroalcoholic extract of Euterpe oleracea Mart. seed in the MCF-7 cell line and to identify the compounds responsible for the antineoplastic action. METHODS: Cells were treated with 10, 20, 40 and 60 µg/ml with the hexane, chloroform and ethyl acetate fraction (EAF) of the hydroalcoholic extract of açaí seed, for 24 and 48 h. After treatment, cell viability was measured using MTT assay and cell death was assessed using the Annexin-Pi assay. The most cytotoxic fraction under study was analysed by mass spectrometry using an electrospray ionization source and a cyclotron analyser coupled to a Fourier transform. Data were analysed statistically by analysis of variance (ANOVA) or by Student's t-test, where appropriate. KEY FINDINGS: All fractions caused significant reduction in the cell viability, but the EAF was the most cytotoxic (P < 0.001). It was observed the absence of significant annexin staining but increase Pi staining (P < 0.001). The EAF is composed of epicatechin, proanthocyanidin A2 and trimeric and tetrameric procyanidins. CONCLUSIONS: In this study, we demonstrated that EAF was the most effective fraction in reducing cell viability and causing necroptosis in the MCF-7 cell.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Extratos Vegetais / Euterpe Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: J Pharm Pharmacol Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Extratos Vegetais / Euterpe Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: J Pharm Pharmacol Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido