Dietary soy protein improves adipose tissue dysfunction by modulating parameters related with oxidative stress in dyslipidemic insulin-resistant rats.

Illesca, Paola G; Álvarez, Silvina M; Selenscig, Dante A; Ferreira, María Del R; Giménez, María S; Lombardo, Yolanda B; D'Alessandro, María E

Illesca, Paola G; Álvarez, Silvina M; Selenscig, Dante A; Ferreira, María Del R; Giménez, María S; Lombardo, Yolanda B; D'Alessandro, María E.

Afiliação

Illesca PG; Department of Biochemistry, School of Biochemistry, University of Litoral, Ciudad Universitaria, El Pozo CC 242, 3000, Santa Fe, Argentina.
Álvarez SM; Laboratory of Molecular Biochemistry, Faculty of Chemistry, Biochemistry and Pharmacy, National University of San Luis, Avenida Ejercito de los Andes 950, 5700, San Luis, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina.
Selenscig DA; Department of Biochemistry, School of Biochemistry, University of Litoral, Ciudad Universitaria, El Pozo CC 242, 3000, Santa Fe, Argentina.
Ferreira MDR; Department of Biochemistry, School of Biochemistry, University of Litoral, Ciudad Universitaria, El Pozo CC 242, 3000, Santa Fe, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina.
Giménez MS; Laboratory of Molecular Biochemistry, Faculty of Chemistry, Biochemistry and Pharmacy, National University of San Luis, Avenida Ejercito de los Andes 950, 5700, San Luis, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina.
Lombardo YB; Department of Biochemistry, School of Biochemistry, University of Litoral, Ciudad Universitaria, El Pozo CC 242, 3000, Santa Fe, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina.
D'Alessandro ME; Department of Biochemistry, School of Biochemistry, University of Litoral, Ciudad Universitaria, El Pozo CC 242, 3000, Santa Fe, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina. Electronic address: medaless@fbcb.unl.edu.ar.

Biomed Pharmacother ; 88: 1008-1015, 2017 Apr.

Article em En | MEDLINE | ID: mdl-28178612

RESUMO

The present study investigates the benefits of the dietary intake of soy protein on adipose tissue dysfunction in a rat model that mimics several aspects of the human metabolic syndrome. Wistar rats were fed a sucrose-rich diet (SRD) for 4 months. After that, half of the animals continued with SRD until month 8 while in the other half, casein protein was replaced by isolated soy protein for 4 months (SRD-S). A reference group consumed a control diet all the time. In adipose tissue we determined: i) the activities of antioxidant enzymes, gene expression of Mn-superoxide dismutase (SOD) and glutathione peroxidase (GPx), and glutathione redox state ii) the activity of xanthine oxidase (XO), ROS levels and the gene expression of NAD(P)H oxidase iii) the expression of the nuclear factor erythroid-2 related factor-2 (Nrf2). Besides, adiposity visceral index, insulin sensitivity, and tumor necrosis factor-α (TNF-α) in plasma were determined. Compared with the SRD-fed rats, the animals fed a SRD-S showed: activity normalization of SOD and glutathione reductase, improvement of mRNA SOD and normalization of mRNA GPx without changes in the expression of the Nrf2, and improvement of glutathione redox state. These results were accompanied by a normalization of XO activity and improvement of both the ROS production as well as TNF-α levels in plasma. Besides, adipocyte size distribution, adiposity visceral index and insulin sensitivity improved. The results suggest that soy protein can be a complementary nutrient for treating some signs of the metabolic syndrome.

Assuntos

Tecido Adiposo/patologia; Tecido Adiposo/fisiopatologia; Proteínas Alimentares/uso terapêutico; Dislipidemias/tratamento farmacológico; Dislipidemias/fisiopatologia; Insulina/metabolismo; Estresse Oxidativo; Proteínas de Soja/uso terapêutico; Adipócitos/efeitos dos fármacos; Adipócitos/metabolismo; Adipócitos/patologia; Tecido Adiposo/efeitos dos fármacos; Adiposidade/efeitos dos fármacos; Animais; Antioxidantes/metabolismo; Biomarcadores/metabolismo; Peso Corporal/efeitos dos fármacos; Proteínas Alimentares/farmacologia; Sacarose Alimentar; Dislipidemias/sangue; Metabolismo Energético/efeitos dos fármacos; Epididimo/efeitos dos fármacos; Epididimo/metabolismo; Glucose/administração & dosagem; Glutationa Peroxidase/genética; Glutationa Peroxidase/metabolismo; Masculino; Fator 2 Relacionado a NF-E2/metabolismo; Tamanho do Órgão/efeitos dos fármacos; Estresse Oxidativo/efeitos dos fármacos; Carbonilação Proteica/efeitos dos fármacos; RNA Mensageiro/genética; RNA Mensageiro/metabolismo; Ratos Wistar; Proteínas de Soja/farmacologia; Superóxido Dismutase/genética; Superóxido Dismutase/metabolismo; Substâncias Reativas com Ácido Tiobarbitúrico/metabolismo; Fator de Necrose Tumoral alfa/sangue

Palavras-chave

Adipose tissue; Antioxidant defenses; High sucrose diet; Oxidative stress; Soy protein; Visceral adiposity

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas Alimentares / Tecido Adiposo / Estresse Oxidativo / Proteínas de Soja / Dislipidemias / Insulina Tipo de estudo: Prognostic_studies Idioma: En Revista: Biomed Pharmacother Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Argentina País de publicação: França

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