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Metabolomic Strategies Involving Mass Spectrometry Combined with Liquid and Gas Chromatography.
Lopes, Aline Soriano; Cruz, Elisa Castañeda Santa; Sussulini, Alessandra; Klassen, Aline.
Afiliação
  • Lopes AS; Department of Exact and Earth Sciences, Federal University of São Paulo (UNIFESP), Diadema, SP, Brazil.
  • Cruz EC; Laboratory of Bioanalytics and Integrated Omics (LaBIOmics), University of Campinas (UNICAMP), Campinas, SP, Brazil.
  • Sussulini A; Laboratory of Bioanalytics and Integrated Omics (LaBIOmics), University of Campinas (UNICAMP), Campinas, SP, Brazil.
  • Klassen A; Department of Exact and Earth Sciences, Federal University of São Paulo (UNIFESP), Diadema, SP, Brazil. aline.klassen@unifesp.br.
Adv Exp Med Biol ; 965: 77-98, 2017.
Article em En | MEDLINE | ID: mdl-28132177
Amongst all omics sciences, there is no doubt that metabolomics is undergoing the most important growth in the last decade. The advances in analytical techniques and data analysis tools are the main factors that make possible the development and establishment of metabolomics as a significant research field in systems biology. As metabolomic analysis demands high sensitivity for detecting metabolites present in low concentrations in biological samples, high-resolution power for identifying the metabolites and wide dynamic range to detect metabolites with variable concentrations in complex matrices, mass spectrometry is being the most extensively used analytical technique for fulfilling these requirements. Mass spectrometry alone can be used in a metabolomic analysis; however, some issues such as ion suppression may difficultate the quantification/identification of metabolites with lower concentrations or some metabolite classes that do not ionise as well as others. The best choice is coupling separation techniques, such as gas or liquid chromatography, to mass spectrometry, in order to improve the sensitivity and resolution power of the analysis, besides obtaining extra information (retention time) that facilitates the identification of the metabolites, especially when considering untargeted metabolomic strategies. In this chapter, the main aspects of mass spectrometry (MS), liquid chromatography (LC) and gas chromatography (GC) are discussed, and recent clinical applications of LC-MS and GC-MS are also presented.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Espectrometria de Massas / Cromatografia Líquida / Metabolômica / Cromatografia Gasosa-Espectrometria de Massas Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: Adv Exp Med Biol Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Espectrometria de Massas / Cromatografia Líquida / Metabolômica / Cromatografia Gasosa-Espectrometria de Massas Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: Adv Exp Med Biol Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos