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Translocation of Bacillus thuringiensis in Phaseolus vulgaris tissues and vertical transmission in Arabidopsis thaliana.
García-Suárez, R; Verduzco-Rosas, L A; Del Rincón-Castro, M C; Délano-Frier, J P; Ibarra, J E.
Afiliação
  • García-Suárez R; Departamento de Biotecnología y Bioquímica, Centro de Investigación y de Estudios Avanzados del IPN, Irapuato, Gto, Mexico.
  • Verduzco-Rosas LA; Departamento de Biotecnología y Bioquímica, Centro de Investigación y de Estudios Avanzados del IPN, Irapuato, Gto, Mexico.
  • Del Rincón-Castro MC; División de Ciencias de la Vida, Departamento de Alimentos, Universidad de Guanajuato, Irapuato, Gto, Mexico.
  • Délano-Frier JP; Departamento de Biotecnología y Bioquímica, Centro de Investigación y de Estudios Avanzados del IPN, Irapuato, Gto, Mexico.
  • Ibarra JE; Departamento de Biotecnología y Bioquímica, Centro de Investigación y de Estudios Avanzados del IPN, Irapuato, Gto, Mexico.
J Appl Microbiol ; 122(4): 1092-1100, 2017 Apr.
Article em En | MEDLINE | ID: mdl-28129468
AIMS: To demonstrate the ability of Bacillus thuringiensis to penetrate as spore-crystal complex to the internal tissues of bean plants, and keep its insecticidal activity. To test the vertical transmission of the spore-crystal complex in Arabidopsis thaliana. METHODS AND RESULTS: The experimental strain was transformed with the pMUTIN-gfp plasmid which labelled the spores of B. thuringiensis HD-73 with the GFP protein. Once the rhizosphere of the bean plants was inoculated with the labelled strain, the bacterium was recovered from leaves, stems, and petioles. Furthermore, toxicity of treated plants was significantly higher than control plants when bio-assayed on cabbage looper larvae. The labelled strain was recovered from the dead insects. When the rhizosphere of A. thaliana plants was inoculated with the labelled strain, mature seeds from these plants were surface-sterilized and grown under in vitro conditions. The labelled strain was recovered from the seedlings. CONCLUSIONS: We showed that B. thuringiensis subsp. kurstaki (HD-73) in the rhizosphere can translocate to upper tissues of bean plants, and keep its insecticidal activity. Transmission of the labelled B. thuringiensis strain passed to the next generation of A. thaliana. SIGNIFICANCE AND IMPACT OF THE STUDY: The role of B. thuringiensis as a potential facultative endophyte bacterium and the possible biotechnological repercussions are discussed.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bacillus thuringiensis / Proteínas de Bactérias / Arabidopsis / Phaseolus / Endotoxinas / Proteínas Hemolisinas / Inseticidas Limite: Animals Idioma: En Revista: J Appl Microbiol Assunto da revista: MICROBIOLOGIA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: México País de publicação: Reino Unido
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bacillus thuringiensis / Proteínas de Bactérias / Arabidopsis / Phaseolus / Endotoxinas / Proteínas Hemolisinas / Inseticidas Limite: Animals Idioma: En Revista: J Appl Microbiol Assunto da revista: MICROBIOLOGIA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: México País de publicação: Reino Unido