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Analysis of Scanning Electron Microscopy Images To Investigate Adsorption Processes Responsible for Detection of Cancer Biomarkers.
Rodrigues, Valquiria da Cruz; Comin, Cesar H; Soares, Juliana Coatrini; Soares, Andrey Coatrini; Melendez, Matias Eliseo; Fregnani, José Humberto T G; Carvalho, André Lopes; Costa, Luciano da F; Oliveira, Osvaldo N.
Afiliação
  • Rodrigues VD; São Carlos Institute of Physics, University of São Paulo , 13560-970 São Carlos, São Paulo, Brazil.
  • Comin CH; São Carlos Institute of Physics, University of São Paulo , 13560-970 São Carlos, São Paulo, Brazil.
  • Soares JC; São Carlos Institute of Physics, University of São Paulo , 13560-970 São Carlos, São Paulo, Brazil.
  • Soares AC; São Carlos Institute of Physics, University of São Paulo , 13560-970 São Carlos, São Paulo, Brazil.
  • Melendez ME; Departament of Materials Engineering, São Carlos School of Engineering, University of São Paulo , 13563-120 São Carlos, São Paulo, Brazil.
  • Fregnani JH; Molecular Oncology Research Center, Barretos Cancer Hospital , 14784-400 Barretos, São Paulo, Brazil.
  • Carvalho AL; Molecular Oncology Research Center, Barretos Cancer Hospital , 14784-400 Barretos, São Paulo, Brazil.
  • Costa LD; Molecular Oncology Research Center, Barretos Cancer Hospital , 14784-400 Barretos, São Paulo, Brazil.
  • Oliveira ON; São Carlos Institute of Physics, University of São Paulo , 13560-970 São Carlos, São Paulo, Brazil.
ACS Appl Mater Interfaces ; 9(7): 5885-5890, 2017 Feb 22.
Article em En | MEDLINE | ID: mdl-28117964
Adsorption processes are responsible for detection of cancer biomarkers in biosensors (and immunosensors), which can be captured with various principles of detection. In this study, we used a biosensor made with nanostructured films of polypyrrole and p53 antibodies, and image analysis of scanning electron microscopy data made it possible to correlate morphological changes of the biosensor with the concentration of cells containing the cancer biomarker p53. The selectivity of the biosensor was proven by distinguishing images obtained with exposure of the biosensor to cells containing the biomarker from those acquired with cells that did not contain it. Detection was confirmed with cyclic voltammetry measurements, while the adsorption of the p53 biomarker was probed with polarization-modulated infrared reflection absorption (PM-IRRAS) and a quartz crystal microbalance (QCM). Adsorption is described using the Langmuir-Freundlich model, with saturation taking place at a concentration of 100 Ucells/mL. Taken together, our results point to novel ways to detect biomarkers or any type of analyte for which detection is based on adsorption as is the case of the majority of biosensors.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Biomarcadores Tumorais Tipo de estudo: Diagnostic_studies Idioma: En Revista: ACS Appl Mater Interfaces Assunto da revista: BIOTECNOLOGIA / ENGENHARIA BIOMEDICA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Biomarcadores Tumorais Tipo de estudo: Diagnostic_studies Idioma: En Revista: ACS Appl Mater Interfaces Assunto da revista: BIOTECNOLOGIA / ENGENHARIA BIOMEDICA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos