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Isolation and biological and molecular characterization of Neospora caninum (NC-SP1) from a naturally infected adult asymptomatic cattle (Bos taurus) in the state of São Paulo, Brazil.
Oliveira, Solange; Soares, Rodrigo Martins; Aizawa, Juliana; Soares, Herbert Sousa; Chiebao, Daniela Pontes; Ortega-Mora, Luis Miguel; Regidor-Cerrillo, Javier; Silva, Natália Quadros Bressa; Gennari, Solange Maria; Pena, Hilda Fátima Jesus.
Afiliação
  • Oliveira S; Department of Preventive Veterinary Medicine and Animal Health,Faculty of Veterinary Medicine,University of São Paulo,Av. Prof. Dr. Orlando Marques de Paiva, 87, CEP 05508-270, São Paulo,Brazil.
  • Soares RM; Department of Preventive Veterinary Medicine and Animal Health,Faculty of Veterinary Medicine,University of São Paulo,Av. Prof. Dr. Orlando Marques de Paiva, 87, CEP 05508-270, São Paulo,Brazil.
  • Aizawa J; Department of Preventive Veterinary Medicine and Animal Health,Faculty of Veterinary Medicine,University of São Paulo,Av. Prof. Dr. Orlando Marques de Paiva, 87, CEP 05508-270, São Paulo,Brazil.
  • Soares HS; Department of Preventive Veterinary Medicine and Animal Health,Faculty of Veterinary Medicine,University of São Paulo,Av. Prof. Dr. Orlando Marques de Paiva, 87, CEP 05508-270, São Paulo,Brazil.
  • Chiebao DP; Department of Preventive Veterinary Medicine and Animal Health,Faculty of Veterinary Medicine,University of São Paulo,Av. Prof. Dr. Orlando Marques de Paiva, 87, CEP 05508-270, São Paulo,Brazil.
  • Ortega-Mora LM; SALUVET, AnimalHealth Department,Faculty of Veterinary Sciences, Complutense University of Madrid, Ciudad Universitaria s/n,28040 Madrid,Spain.
  • Regidor-Cerrillo J; SALUVET, AnimalHealth Department,Faculty of Veterinary Sciences, Complutense University of Madrid, Ciudad Universitaria s/n,28040 Madrid,Spain.
  • Silva NQB; Department of Preventive Veterinary Medicine and Animal Health,Faculty of Veterinary Medicine,University of São Paulo,Av. Prof. Dr. Orlando Marques de Paiva, 87, CEP 05508-270, São Paulo,Brazil.
  • Gennari SM; Department of Preventive Veterinary Medicine and Animal Health,Faculty of Veterinary Medicine,University of São Paulo,Av. Prof. Dr. Orlando Marques de Paiva, 87, CEP 05508-270, São Paulo,Brazil.
  • Pena HFJ; Department of Preventive Veterinary Medicine and Animal Health,Faculty of Veterinary Medicine,University of São Paulo,Av. Prof. Dr. Orlando Marques de Paiva, 87, CEP 05508-270, São Paulo,Brazil.
Parasitology ; 144(6): 707-711, 2017 05.
Article em En | MEDLINE | ID: mdl-28073388
The biological and genetic diversity of Neospora caninum is very limited because of availability of only a few viable isolates worldwide. This study describes the isolation and biological and molecular characterization of a new viable isolate of N. caninum (NC-SP1), from a cattle in Brazil. Approximately 400 g of brain from a naturally infected adult male cattle from an abattoir was fed to a 2-month-old dog. Neospora-like oocysts were observed on day 7 post-inoculation (PI) and the duration of oocyst shedding was 14 days. The DNA obtained from oocysts was characterized molecularly and the final sequence was 99% identical to homologous sequences of N. caninum available in GenBank®. For bioassay, gerbils (Meriones unguiculatus) were orally inoculated with 10 100 and 1000 oocysts; all gerbils remained clinically normal but developed N. caninum antibodies 14 days PI. Cell culture isolation was successful using the brain homogenate from one of the gerbils and tachyzoites were observed 24 days PI. Microsatellite genotyping revealed a unique genetic profile for this new reference isolate.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doenças dos Bovinos / Coccidiose / Neospora Limite: Animals País/Região como assunto: America do sul / Brasil Idioma: En Revista: Parasitology Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doenças dos Bovinos / Coccidiose / Neospora Limite: Animals País/Região como assunto: America do sul / Brasil Idioma: En Revista: Parasitology Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido