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Isolation of Microglia and Immune Infiltrates from Mouse and Primate Central Nervous System.
Galatro, Thais F; Vainchtein, Ilia D; Brouwer, Nieske; Boddeke, Erik W G M; Eggen, Bart J L.
Afiliação
  • Galatro TF; Department of Neurology, School of Medicine, University of São Paulo, São Paulo, 01246903, Brazil.
  • Vainchtein ID; Department of Neuroscience, Section Medical Physiology, University of Groningen, University Medical Center Groningen, Groningen, 9713 AV, The Netherlands.
  • Brouwer N; Department of Neuroscience, Section Medical Physiology, University of Groningen, University Medical Center Groningen, Groningen, 9713 AV, The Netherlands.
  • Boddeke EWGM; Department of Neuroscience, Section Medical Physiology, University of Groningen, University Medical Center Groningen, Groningen, 9713 AV, The Netherlands.
  • Eggen BJL; Department of Neuroscience, Section Medical Physiology, University of Groningen, University Medical Center Groningen, Groningen, 9713 AV, The Netherlands. b.j.l.eggen@umcg.nl.
Methods Mol Biol ; 1559: 333-342, 2017.
Article em En | MEDLINE | ID: mdl-28063055
Microglia are the innate immune cells of the central nervous system (CNS) and play an important role in the maintenance of tissue homeostasis, providing neural support and neuroprotection. Microglia constantly survey their environment and quickly respond to homeostatic perturbations. Microglia are increasingly implicated in neuropathological and neurodegenerative conditions, such as Alzheimer's disease, Parkinson's disease, and glioma progression. Here, we describe a detailed isolation protocol for microglia and immune infiltrates, optimized for large amounts of post mortem tissue from human and rhesus macaque, as well as smaller tissue amounts from mouse brain and spinal cord, that yield a highly purified microglia population (up to 98 % purity). This acute isolation protocol is based on mechanical dissociation and a two-step density gradient purification, followed by fluorescence-activated cell sorting (FACS) to obtain pure microglia and immune infiltrate populations.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias Encefálicas / Separação Celular / Microglia / Doenças Neurodegenerativas / Citometria de Fluxo / Glioma Limite: Animals / Humans Idioma: En Revista: Methods Mol Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias Encefálicas / Separação Celular / Microglia / Doenças Neurodegenerativas / Citometria de Fluxo / Glioma Limite: Animals / Humans Idioma: En Revista: Methods Mol Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos