Your browser doesn't support javascript.
loading
Protease Inhibitors Extracted from Caesalpinia echinata Lam. Affect Kinin Release during Lung Inflammation.
Cruz-Silva, Ilana; Nunes, Viviane Abreu; Gozzo, Andrezza Justino; Praxedes-Garcia, Priscila; Tanaka, Aparecida Sadae; Shimamoto, Kazuaki; Araujo, Mariana Silva.
Afiliação
  • Cruz-Silva I; Department of Biochemistry, Universidade Federal de São Paulo, Rua Três de Maio, No. 100, 04044-020 São Paulo, SP, Brazil.
  • Nunes VA; School of Arts, Sciences and Humanities, Universidade de São Paulo, Avenida Arlindo Bettio, No. 1000, 03828-000 São Paulo, SP, Brazil.
  • Gozzo AJ; Department of Marine Sciences, Universidade Federal de São Paulo, Rua Doutor Carvalho de Mendonça, No. 144, 11070-100 Santos, SP, Brazil.
  • Praxedes-Garcia P; Department of Biochemistry, Universidade Federal de São Paulo, Rua Três de Maio, No. 100, 04044-020 São Paulo, SP, Brazil.
  • Tanaka AS; Department of Biochemistry, Universidade Federal de São Paulo, Rua Três de Maio, No. 100, 04044-020 São Paulo, SP, Brazil.
  • Shimamoto K; Japan Health Care College, Sinei 434-1, Kiyota-ku, Sapporo, Japan.
  • Araujo MS; Department of Biochemistry, Universidade Federal de São Paulo, Rua Três de Maio, No. 100, 04044-020 São Paulo, SP, Brazil.
Pulm Med ; 2016: 9425807, 2016.
Article em En | MEDLINE | ID: mdl-28044105
Inflammation is an essential process in many pulmonary diseases in which kinins are generated by protease action on kininogen, a phenomenon that is blocked by protease inhibitors. We evaluated kinin release in an in vivo lung inflammation model in rats, in the presence or absence of CeKI (C. echinata kallikrein inhibitor), a plasma kallikrein, cathepsin G, and proteinase-3 inhibitor, and rCeEI (recombinant C. echinata elastase inhibitor), which inhibits these proteases and also neutrophil elastase. Wistar rats were intravenously treated with buffer (negative control) or inhibitors and, subsequently, lipopolysaccharide was injected into their lungs. Blood, bronchoalveolar lavage fluid (BALF), and lung tissue were collected. In plasma, kinin release was higher in the LPS-treated animals in comparison to CeKI or rCeEI groups. rCeEI-treated animals presented less kinin than CeKI-treated group. Our data suggest that kinins play a pivotal role in lung inflammation and may be generated by different enzymes; however, neutrophil elastase seems to be the most important in the lung tissue context. These results open perspectives for a better understanding of biological process where neutrophil enzymes participate and indicate these plant inhibitors and their recombinant correlates for therapeutic trials involving pulmonary diseases.
Assuntos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pneumonia / Caesalpinia / Neutrófilos Limite: Animals Idioma: En Revista: Pulm Med Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Brasil País de publicação: Egito
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pneumonia / Caesalpinia / Neutrófilos Limite: Animals Idioma: En Revista: Pulm Med Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Brasil País de publicação: Egito