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GENETIC MUTATIONS AFFECTING THE FIRST LINE ERADICATION THERAPY OF Helicobacter pylori-INFECTED EGYPTIAN PATIENTS.
Ramzy, Iman; Elgarem, Hassan; Hamza, Iman; Ghaith, Doaa; Elbaz, Tamer; Elhosary, Waleed; Mostafa, Gehan; Elzahry, Mohammad A Mohey Eldin.
Afiliação
  • Ramzy I; Endemic Medicine and Hepatology Department, Faculty of Medicine, Cairo University, Cairo, Egypt. E-mails: hgarem@yahoo.cpm; Iman_hamza@hotmail.com; Iman_ramzy@hotmail.com; ma_mohey@kasralainy.edu.eg; Wh8609@gmail.com; gehan1511@gmail.com; tamerbaz@yahoo.com.
  • Elgarem H; Endemic Medicine and Hepatology Department, Faculty of Medicine, Cairo University, Cairo, Egypt. E-mails: hgarem@yahoo.cpm; Iman_hamza@hotmail.com; Iman_ramzy@hotmail.com; ma_mohey@kasralainy.edu.eg; Wh8609@gmail.com; gehan1511@gmail.com; tamerbaz@yahoo.com.
  • Hamza I; Endemic Medicine and Hepatology Department, Faculty of Medicine, Cairo University, Cairo, Egypt. E-mails: hgarem@yahoo.cpm; Iman_hamza@hotmail.com; Iman_ramzy@hotmail.com; ma_mohey@kasralainy.edu.eg; Wh8609@gmail.com; gehan1511@gmail.com; tamerbaz@yahoo.com.
  • Ghaith D; Clinical and Chemical Pathology Department, Faculty of Medicine, Cairo University, Cairo, Egypt. E-mail: doaa.ghaith@kasralainy.edu.eg.
  • Elbaz T; Endemic Medicine and Hepatology Department, Faculty of Medicine, Cairo University, Cairo, Egypt. E-mails: hgarem@yahoo.cpm; Iman_hamza@hotmail.com; Iman_ramzy@hotmail.com; ma_mohey@kasralainy.edu.eg; Wh8609@gmail.com; gehan1511@gmail.com; tamerbaz@yahoo.com.
  • Elhosary W; Endemic Medicine and Hepatology Department, Faculty of Medicine, Cairo University, Cairo, Egypt. E-mails: hgarem@yahoo.cpm; Iman_hamza@hotmail.com; Iman_ramzy@hotmail.com; ma_mohey@kasralainy.edu.eg; Wh8609@gmail.com; gehan1511@gmail.com; tamerbaz@yahoo.com.
  • Mostafa G; Endemic Medicine and Hepatology Department, Faculty of Medicine, Cairo University, Cairo, Egypt. E-mails: hgarem@yahoo.cpm; Iman_hamza@hotmail.com; Iman_ramzy@hotmail.com; ma_mohey@kasralainy.edu.eg; Wh8609@gmail.com; gehan1511@gmail.com; tamerbaz@yahoo.com.
  • Elzahry MA; Endemic Medicine and Hepatology Department, Faculty of Medicine, Cairo University, Cairo, Egypt. E-mails: hgarem@yahoo.cpm; Iman_hamza@hotmail.com; Iman_ramzy@hotmail.com; ma_mohey@kasralainy.edu.eg; Wh8609@gmail.com; gehan1511@gmail.com; tamerbaz@yahoo.com.
Rev Inst Med Trop Sao Paulo ; 58: 88, 2016 Dec 08.
Article em En | MEDLINE | ID: mdl-27982354
INTRODUCTION:: Several genetic mutations affect the first-line triple therapy for Helicobacter pylori. We aimed to study the most common genetic mutations affecting the metronidazole and clarithromycin therapy for H. pylori-infected Egyptian patients. PATIENTS AND METHODS:: In our study, we included 100 successive dyspeptic patients scheduled for diagnosis through upper gastroscopy at Cairo's University Hospital, Egypt. Gastric biopsies were tested for the presence of H. pylori by detection of the 16S rRNA gene. Positive biopsies were further studied for the presence of the rdxA gene deletion by Polymerase Chain Reaction (PCR), while clarithromycin resistance was investigated by the presence of nucleotide substitutions within H. pylori 23S rRNA V domain using MboII and BsaI to carry out a Restricted Fragment Length Polymorphism (RFLP) assay. RESULTS:: Among 70 H. pylori positive biopsies, the rdxA gene deletion was detected in 44/70 (62.9%) samples, while predominance of the A2142G mutations within the H. pylori 23S rRNA V domain was evidenced in 39/70 (55.7%) of the positive H. pylori cases. No statistically significant difference was found between the presence of gene mutations and different factors such as patients 'age, gender, geographic distribution, symptoms and endoscopic findings. CONCLUSION:: Infection with mutated H. pylori strains is considerably high, a finding that imposes care in the use of the triple therapy to treat H. pylori in Egypt, since the guidelines recommend to abandon the standard triple therapy when the primary clarithromycin resistance rate is over 20%1.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Helicobacter pylori / Infecções por Helicobacter / Claritromicina / Farmacorresistência Bacteriana / Metronidazol / Mutação Tipo de estudo: Guideline Limite: Adult / Female / Humans / Male / Middle aged País/Região como assunto: Africa Idioma: En Revista: Rev Inst Med Trop Sao Paulo Ano de publicação: 2016 Tipo de documento: Article País de publicação: Brasil
Texto completo
Adicionar na Minha BVS
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XML
PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Helicobacter pylori / Infecções por Helicobacter / Claritromicina / Farmacorresistência Bacteriana / Metronidazol / Mutação Tipo de estudo: Guideline Limite: Adult / Female / Humans / Male / Middle aged País/Região como assunto: Africa Idioma: En Revista: Rev Inst Med Trop Sao Paulo Ano de publicação: 2016 Tipo de documento: Article País de publicação: Brasil