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Neural Differentiation of Mesenchymal Stem Cells on Scaffolds for Nerve Tissue Engineering Applications.
Quintiliano, Kerlin; Crestani, Thayane; Silveira, Davi; Helfer, Virginia Etges; Rosa, Annelise; Balbueno, Eduardo; Steffens, Daniela; Jotz, Geraldo Pereira; Pilger, Diogo André; Pranke, Patricia.
Afiliação
  • Quintiliano K; 1 Hematology and Stem Cell Laboratory, Faculty of Pharmacy, Universidade Federal do Rio Grande do Sul , Porto Alegre, Brazil .
  • Crestani T; 2 Post-graduate Program in Neuroscience, Universidade Federal do Rio Grande do Sul , Porto Alegre, Brazil .
  • Silveira D; 1 Hematology and Stem Cell Laboratory, Faculty of Pharmacy, Universidade Federal do Rio Grande do Sul , Porto Alegre, Brazil .
  • Helfer VE; 2 Post-graduate Program in Neuroscience, Universidade Federal do Rio Grande do Sul , Porto Alegre, Brazil .
  • Rosa A; 1 Hematology and Stem Cell Laboratory, Faculty of Pharmacy, Universidade Federal do Rio Grande do Sul , Porto Alegre, Brazil .
  • Steffens D; 1 Hematology and Stem Cell Laboratory, Faculty of Pharmacy, Universidade Federal do Rio Grande do Sul , Porto Alegre, Brazil .
  • Jotz GP; 3 Post-graduate Program in Material Science, Universidade Federal do Rio Grande do Sul , Porto Alegre, Brazil .
  • Pilger DA; 1 Hematology and Stem Cell Laboratory, Faculty of Pharmacy, Universidade Federal do Rio Grande do Sul , Porto Alegre, Brazil .
  • Pranke P; 1 Hematology and Stem Cell Laboratory, Faculty of Pharmacy, Universidade Federal do Rio Grande do Sul , Porto Alegre, Brazil .
Cell Reprogram ; 18(6): 369-381, 2016 11.
Article em En | MEDLINE | ID: mdl-27906586
Scaffolds produced by electrospinning act as supports for cell proliferation and differentiation, improved through the release of neurotrophic factors. The objective of this study was to develop aligned and random nanofiber scaffolds with and without nerve growth factor to evaluate the potential of mesenchymal stem cells (MSCs) for neural differentiation. Nanofiber morphology, diameter, degradability, cell morphology, adhesion, proliferation, viability, cytotoxicity, and neural differentiation were performed to characterize the scaffolds. The expression for nestin, ß-III tubulin, and neuron-specific enolase was also evaluated. The scaffolds demonstrated a satisfactory environment for MSC growth, being nontoxic. The MSCs cultivated on the scaffolds were able to adhere and proliferate. The evaluation of neural differentiation indicated that in all groups of scaffolds the MSCs were able to upregulate neural gene expression.
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células-Tronco / Engenharia Tecidual / Polpa Dentária / Alicerces Teciduais / Neurogênese / Células-Tronco Mesenquimais / Neurônios Limite: Humans Idioma: En Revista: Cell Reprogram Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Buscar no Google
Adicionar na Minha BVS
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PubMed Links

Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células-Tronco / Engenharia Tecidual / Polpa Dentária / Alicerces Teciduais / Neurogênese / Células-Tronco Mesenquimais / Neurônios Limite: Humans Idioma: En Revista: Cell Reprogram Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos