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Development and field performance of nitrogen use efficient rice lines for Africa.
Selvaraj, Michael Gomez; Valencia, Milton Orlando; Ogawa, Satoshi; Lu, Yingzhi; Wu, Liying; Downs, Christopher; Skinner, Wayne; Lu, Zhongjin; Kridl, Jean C; Ishitani, Manabu; van Boxtel, Jos.
Afiliação
  • Selvaraj MG; International Center for Tropical Agriculture (CIAT), Cali, Colombia.
  • Valencia MO; International Center for Tropical Agriculture (CIAT), Cali, Colombia.
  • Ogawa S; Faculty of Agriculture Science, National University of Colombia, Palmira, Colombia.
  • Lu Y; International Center for Tropical Agriculture (CIAT), Cali, Colombia.
  • Wu L; Japan Society for the Promotion of Science Postdoctoral Fellow, Graduate School of Agricultural and Life Science, The University of Tokyo, Tokyo, Japan.
  • Downs C; Arcadia Biosciences, Davis, CA, USA.
  • Skinner W; Arcadia Biosciences, Davis, CA, USA.
  • Lu Z; Arcadia Biosciences, Davis, CA, USA.
  • Kridl JC; Arcadia Biosciences, Davis, CA, USA.
  • Ishitani M; Arcadia Biosciences, Davis, CA, USA.
  • van Boxtel J; Arcadia Biosciences, Davis, CA, USA.
Plant Biotechnol J ; 15(6): 775-787, 2017 Jun.
Article em En | MEDLINE | ID: mdl-27889933
Nitrogen (N) fertilizers are a major input cost in rice production, and its excess application leads to major environmental pollution. Development of rice varieties with improved nitrogen use efficiency (NUE) is essential for sustainable agriculture. Here, we report the results of field evaluations of marker-free transgenic NERICA4 (New Rice for Africa 4) rice lines overexpressing barley alanine amino transferase (HvAlaAT) under the control of a rice stress-inducible promoter (pOsAnt1). Field evaluations over three growing seasons and two rice growing ecologies (lowland and upland) revealed that grain yield of pOsAnt1:HvAlaAT transgenic events was significantly higher than sibling nulls and wild-type controls under different N application rates. Our field results clearly demonstrated that this genetic modification can significantly increase the dry biomass and grain yield compared to controls under limited N supply. Increased yield in transgenic events was correlated with increased tiller and panicle number in the field, and evidence of early establishment of a vigorous root system in hydroponic growth. Our results suggest that expression of the HvAlaAT gene can improve NUE in rice without causing undesirable growth phenotypes. The NUE technology described in this article has the potential to significantly reduce the need for N fertilizer and simultaneously improve food security, augment farm economics and mitigate greenhouse gas emissions from the rice ecosystem.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oryza / Nitrogênio Idioma: En Revista: Plant Biotechnol J Assunto da revista: BIOTECNOLOGIA / BOTANICA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Colômbia País de publicação: Reino Unido

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oryza / Nitrogênio Idioma: En Revista: Plant Biotechnol J Assunto da revista: BIOTECNOLOGIA / BOTANICA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Colômbia País de publicação: Reino Unido