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Rapid antigen detection test for respiratory syncytial virus diagnosis as a diagnostic tool.
Mesquita, Flávio da Silva; Oliveira, Danielle Bruna Leal de; Crema, Daniela; Pinez, Célia Miranda Nunes; Colmanetti, Thaís Cristina; Thomazelli, Luciano Matsumia; Gilio, Alfredo Elias; Vieira, Sandra Elisabeth; Martinez, Marina Baquerizo; Botosso, Viviane Fongaro; Durigon, Edison Luiz.
Afiliação
  • Mesquita FDS; Universidade de São Paulo, Instituto de Ciências Biomédicas, Departamento de Microbiologia, São Paulo, SP, Brazil.
  • Oliveira DBL; Universidade de São Paulo, Instituto de Ciências Biomédicas, Departamento de Microbiologia, São Paulo, SP, Brazil. Electronic address: danibruna@gmail.com.
  • Crema D; Universidade de São Paulo, Hospital Universitário, Laboratório Clínico, São Paulo, SP, Brazil.
  • Pinez CMN; Universidade de São Paulo, Hospital Universitário, Laboratório Clínico, São Paulo, SP, Brazil.
  • Colmanetti TC; Universidade de São Paulo, Instituto de Ciências Biomédicas, Departamento de Microbiologia, São Paulo, SP, Brazil.
  • Thomazelli LM; Universidade de São Paulo, Instituto de Ciências Biomédicas, Departamento de Microbiologia, São Paulo, SP, Brazil.
  • Gilio AE; Universidade de São Paulo, Hospital Universitário, Divisão de Pediatria, São Paulo, SP, Brazil.
  • Vieira SE; Universidade de São Paulo, Hospital Universitário, Divisão de Pediatria, São Paulo, SP, Brazil.
  • Martinez MB; Universidade de São Paulo, Hospital Universitário, Laboratório Clínico, São Paulo, SP, Brazil; Universidade de São Paulo, Escola de Ciências Farmacêuticas, São Paulo, SP, Brazil.
  • Botosso VF; Instituto Butantan, Laboratório de Virologia, Divisão de Desenvolvimento Científico, São Paulo, SP, Brazil.
  • Durigon EL; Universidade de São Paulo, Instituto de Ciências Biomédicas, Departamento de Microbiologia, São Paulo, SP, Brazil.
J Pediatr (Rio J) ; 93(3): 246-252, 2017.
Article em En | MEDLINE | ID: mdl-27889321
OBJECTIVE: The aim of this study was to evaluate the QuickVue® RSV Test Kit (QUIDEL Corp, CA, USA) as a screening tool for respiratory syncytial virus in children with acute respiratory disease in comparison with the indirect immunofluorescence assay as gold standard. In Brazil, rapid antigen detection tests for respiratory syncytial virus are not routinely utilized as a diagnostic tool, except for the diagnosis of dengue and influenza. METHODS: The authors retrospectively analyzed 486 nasopharyngeal aspirate samples from children under age 5 with acute respiratory infection, between December 2013 and August 2014, the samples were analyzed by indirect immunofluorescence assay and QuickVue® RSV Test kit. Samples with discordant results were analyzed by real time PCR and nucleotide sequencing. RESULTS: From 313 positive samples by immunofluorescence assays, 282 (90%) were also positive by the rapid antigen detection test, two were positive only by rapid antigen detection test, 33 were positive only by immunofluorescence assays, and 171 were positive by both methods. The 35 samples with discordant results were analyzed by real time PCR; the two samples positive only by rapid antigen detection test and the five positive only by immunofluorescence assays were also positive by real time PCR. There was no relation between the negativity by QuickVue® RSV Test and viral load or specific strain. The QuickVue® RSV Test showed sensitivity of 90%, specificity of 98.8%, predictive positive value of 99.3%, and negative predictive value of 94.6%, with accuracy of 93.2% and agreement κ index of 0.85 in comparison to immunofluorescence assay. CONCLUSIONS: This study demonstrated that the QuickVue® RSV Test Kit can be effective in early detection of Respiratory syncytial virus in nasopharyngeal aspirate and is reliable for use as a diagnostic tool in pediatrics.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Vírus Sinciciais Respiratórios / Infecções por Vírus Respiratório Sincicial / Reação em Cadeia da Polimerase Via Transcriptase Reversa / Antígenos Virais Tipo de estudo: Diagnostic_studies / Observational_studies / Prognostic_studies / Risk_factors_studies / Screening_studies Limite: Child, preschool / Female / Humans / Male País/Região como assunto: America do sul / Brasil Idioma: En Revista: J Pediatr (Rio J) Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Vírus Sinciciais Respiratórios / Infecções por Vírus Respiratório Sincicial / Reação em Cadeia da Polimerase Via Transcriptase Reversa / Antígenos Virais Tipo de estudo: Diagnostic_studies / Observational_studies / Prognostic_studies / Risk_factors_studies / Screening_studies Limite: Child, preschool / Female / Humans / Male País/Região como assunto: America do sul / Brasil Idioma: En Revista: J Pediatr (Rio J) Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil