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Crude extract of cyanobacteria (Radiocystis fernandoi, strain R28) induces liver impairments in fish.
Paulino, M G; Tavares, D; Bieczynski, F; Pedrão, P G; Souza, N E S; Sakuragui, M M; Luquet, C M; Terezan, A P; Fernandes, J B; Giani, A; Fernandes, M N.
Afiliação
  • Paulino MG; Department of Physiological Sciences, Federal University of São Carlos, São Carlos, São Paulo, Brazil.
  • Tavares D; Department of Physiological Sciences, Federal University of São Carlos, São Carlos, São Paulo, Brazil.
  • Bieczynski F; Center of Applied Ecology of Neuquen, INIBIOMA, UNCo-CONICET- Ruta Provincial 61, km 3, 8371, Junín de los Andes, Neuquén, Argentina.
  • Pedrão PG; Department of Physiological Sciences, Federal University of São Carlos, São Carlos, São Paulo, Brazil.
  • Souza NES; Department of Physiological Sciences, Federal University of São Carlos, São Carlos, São Paulo, Brazil.
  • Sakuragui MM; Department of Physiological Sciences, Federal University of São Carlos, São Carlos, São Paulo, Brazil.
  • Luquet CM; Center of Applied Ecology of Neuquen, INIBIOMA, UNCo-CONICET- Ruta Provincial 61, km 3, 8371, Junín de los Andes, Neuquén, Argentina.
  • Terezan AP; Department of Chemistry, Federal University of São Carlos, São Carlos, São Paulo, Brazil.
  • Fernandes JB; Department of Chemistry, Federal University of São Carlos, São Carlos, São Paulo, Brazil.
  • Giani A; Department of Botany, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.
  • Fernandes MN; Department of Physiological Sciences, Federal University of São Carlos, São Carlos, São Paulo, Brazil. Electronic address: dmnf@ufscar.br.
Aquat Toxicol ; 182: 91-101, 2017 Jan.
Article em En | MEDLINE | ID: mdl-27886582
Radiocystis fernandoi R28 strain is a cyanobacterium which produces mostly the RR and YR microcystin variants (MC-RR and MC-YR, respectively). The effects of crude extract of the R. fernandoi strain R28 were evaluated on the protein phosphatases and on the structure and ultrastructure of the liver of the Neotropical fish, Hoplias malabaricus, after acute and subchronic exposure. Concomitantly, the accumulation of the majority of MCs was determined in the liver and muscle. The fish were exposed to 120.60 MC-RR+MC-LR kg-fish-1 (=100µg MC-LReq kg-fish-1) for 12 and 96h (one single dose, acute exposure) and 30days (one similar dose every 72h, subchronic exposure). MCs did not accumulate in the muscle but, in the liver, MC-YR accumulated after acute exposure and MC-RR and MC-YR accumulation occurred after subchronic exposure. Protein phosphatase 2A (PP2A) activity was inhibited only after subchronic exposure. Acute exposure induced liver hyperemia, hemorrhage, changes in hepatocytes and cord-like disorganization. At the ultrastructural level, the decreasing of glycogen and lipid levels, the swelling of mitochondria and whirling of endoplasmic reticulum suggested hepatocyte necrosis. Subchronic exposure resulted in a complete disarrangement of cord-like hepatocytes, some recovery of mitochondria and whirling endoplasmic reticulum and extensive connective tissues containing fibrous materials in the liver parenchyma. Despite microcystin toxicity and liver alterations, no tumor was induced by MCs. In conclusion, the increased algal mass of R. fernandoi in tropical freshwater, producing mainly MC-RR and MC-YR variants, results in fish liver impairments.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cianobactérias / Microcistinas / Peixes / Fígado Limite: Animals Idioma: En Revista: Aquat Toxicol Assunto da revista: BIOLOGIA / TOXICOLOGIA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cianobactérias / Microcistinas / Peixes / Fígado Limite: Animals Idioma: En Revista: Aquat Toxicol Assunto da revista: BIOLOGIA / TOXICOLOGIA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda