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Effects of deletion of the ac109 gene of Autographa californica nucleopolyhedrovirus on interactions with mammalian cells.
Alfonso, Victoria; Amalfi, Sabrina; López, María Gabriela; Taboga, Oscar.
Afiliação
  • Alfonso V; INTA, CONICET, Instituto de Biotecnología, CICVyA, Nicolás Repetto y de los Reseros S/N, Hurlingham, CP 1686, Buenos Aires, Argentina.
  • Amalfi S; INTA, Instituto de Biotecnología, CICVyA, Nicolás Repetto y de los Reseros S/N, Hurlingham, CP 1686, Buenos Aires, Argentina.
  • López MG; INTA, CONICET, Instituto de Biotecnología, CICVyA, Nicolás Repetto y de los Reseros S/N, Hurlingham, CP 1686, Buenos Aires, Argentina.
  • Taboga O; INTA, CONICET, Instituto de Biotecnología, CICVyA, Nicolás Repetto y de los Reseros S/N, Hurlingham, CP 1686, Buenos Aires, Argentina. taboga.oscaralberto@inta.gob.ar.
Arch Virol ; 162(3): 835-840, 2017 Mar.
Article em En | MEDLINE | ID: mdl-27868165
Baculoviruses are able to enter into mammalian cells, where they can express a transgene that is placed under an appropriate promoter, without producing infectious progeny. ORF109 encodes an essential baculovirus protein that participates in the interaction of the baculovirus with mammalian cells. To date, the mechanisms underlying this interaction are not yet known. We demonstrated that although a Ac109 knock out virus maintained its ability to enter into BHK-21 cells, there was a marked reduction in the expression efficiency of the nuclear transgene. Moreover, the amount of free cytoplasmic viral DNA, which was detected by transcription of a reporter gene, was severely diminished. These results suggest Ac109 could be involved in maintaining the integrity of the viral nucleic acid.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas Virais / Deleção de Genes / Nucleopoliedrovírus Limite: Animals Idioma: En Revista: Arch Virol Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Argentina País de publicação: Áustria
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas Virais / Deleção de Genes / Nucleopoliedrovírus Limite: Animals Idioma: En Revista: Arch Virol Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Argentina País de publicação: Áustria