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Casearin D inhibits ERK phosphorylation and induces downregulation of cyclin D1 in HepG2 cells.
Ferreira-Silva, Guilherme Álvaro; Lages, Carla Carolina Lopes; Sartorelli, Patricia; Hasegawa, Flávia Rie; Soares, Marisi Gomes; Ionta, Marisa.
Afiliação
  • Ferreira-Silva GÁ; Institute of Biomedical Sciences, Federal University of Alfenas, Rua Gabriel Monteiro da Silva, 700, 37130-000, Alfenas, MG, Brazil.
  • Lages CCL; Institute of Chemistry, Federal University of Alfenas, Rua Gabriel Monteiro da Silva, 700, 37130-000, Alfenas, MG, Brazil.
  • Sartorelli P; Institute of Environmental Sciences, Chemical and Pharmaceutical, Federal University of Sao Paulo, Diadema, SP, Brazil.
  • Hasegawa FR; Institute of Environmental Sciences, Chemical and Pharmaceutical, Federal University of Sao Paulo, Diadema, SP, Brazil.
  • Soares MG; Institute of Chemistry, Federal University of Alfenas, Rua Gabriel Monteiro da Silva, 700, 37130-000, Alfenas, MG, Brazil.
  • Ionta M; Institute of Biomedical Sciences, Federal University of Alfenas, Rua Gabriel Monteiro da Silva, 700, 37130-000, Alfenas, MG, Brazil. Electronic address: marisaionta@gmail.com.
Toxicol In Vitro ; 38: 27-32, 2017 Feb.
Article em En | MEDLINE | ID: mdl-27806920
Cancer is a public health problem which represents the second cause of death in the world. In this framework, it is necessary to identify novel compounds with antineoplastic potential. Plants are an important source for discovering novel compounds with pharmacological potential. In this study, we aimed to investigate the antiproliferative potential of isolated compounds from Casearia sylvestris on tumor cell lines. Crude extract effectively reduced cell viability of 4 tumor cell lines (HepG2, A549, U251-MG, and HT-144) after 48h treatment. HepG2 and HT-144 were the most responsive cells. Three fractions (aqueous ethanol, n-hexane and ethyl acetate) were tested against HepG2 and HT-144 cells and we observed that compounds with antiproliferative activity were concentrated in n-hexane and ethyl acetate fractions. The casearins A, G and J were isolated from n-hexane fraction, while casearin D was obtained from ethyl acetate fraction. We demonstrated that casearin D significantly inhibited the clonogenic capacity of HepG2 cells after 24h exposure indicating its antiproliferative activity. In addition, G1/S transition cell cycle arrest in HepG2 cells was also observed. These effects are related, at least in part, to ability of the casearin D in reducing ERK phosphorylation and cyclin D1 expression levels.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ciclina D1 / Diterpenos Clerodânicos / MAP Quinases Reguladas por Sinal Extracelular / Antineoplásicos Limite: Humans Idioma: En Revista: Toxicol In Vitro Assunto da revista: TOXICOLOGIA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ciclina D1 / Diterpenos Clerodânicos / MAP Quinases Reguladas por Sinal Extracelular / Antineoplásicos Limite: Humans Idioma: En Revista: Toxicol In Vitro Assunto da revista: TOXICOLOGIA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido