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Detection of Leishmania (L.) infantum in stray dogs by molecular techniques with sensitive species-specific primers.
Silva, Rodrigo C; Richini-Pereira, Virgínia B; Kikuti, Mariana; Marson, Pâmela M; Langoni, Helio.
Afiliação
  • Silva RC; a Department of Pathobiology and Population Medicine, College of Veterinary Medicine , Mississippi State University , Mississippi State , MS , USA.
  • Richini-Pereira VB; b Regional Laboratory of Bauru , Adolfo Lutz Institute , Bauru , Brazil.
  • Kikuti M; c Institute of Collective Health, Federal University of Bahia , Salvador , Brazil.
  • Marson PM; d Department of Veterinary Hygiene and Public Health, School of Veterinary Medicine and Animal Science , UNESP Universidade Estadual Paulista , Botucatu , Brazil.
  • Langoni H; d Department of Veterinary Hygiene and Public Health, School of Veterinary Medicine and Animal Science , UNESP Universidade Estadual Paulista , Botucatu , Brazil.
Vet Q ; 37(1): 23-30, 2017 Dec.
Article em En | MEDLINE | ID: mdl-27774854
BACKGROUND: Canine visceral leishmaniasis (CVL) is a worldwide parasitic zoonosis caused by Leishmania (Leishmania) infantum around the world. Canids are the definitive hosts and sand flies the intermediate hosts. OBJECTIVE: To test the hypothesis that a new species-specific primers (Lch14:Lch15, targeting a multiple alignment for L. infantum kDNA minicircle) is an efficient diagnostic tool for L. infantum. METHODS: The presence of L. infantum DNA was assessed in blood samples of 69 stray dogs using the conventional PCR (cPCR) and quantitative PCR (qPCR). Additional 50 lymph nodes and 50 bone marrow samples (positive and negative samples for parasitological tests) from dogs from endemic and nonendemic areas for CVL were also used. RESULTS: L. infantum strains, and all positive lymph node and bone marrow samples for parasitological test gave positive results for cPCR and qPCR, presenting analytical sensitivity of ∼100 parasite mL-1. For the blood samples, 40/69 (58%; CI 95%; 46%-69%) resulted positive for L. infantum in both tests. All positive samples were confirmed by sequencing. CONCLUSION: This study showed the importance of the specific detection of L. infantum based on species-specific primers by molecular techniques, highlighting the application as a confirmation method in epidemiological studies and to adopt the best control measures.
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Leishmania infantum / Doenças do Cão / Reação em Cadeia da Polimerase em Tempo Real / Leishmaniose Visceral Tipo de estudo: Diagnostic_studies / Prevalence_studies / Risk_factors_studies Limite: Animals País/Região como assunto: America do sul / Brasil Idioma: En Revista: Vet Q Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Estados Unidos País de publicação: Reino Unido
Buscar no Google
Adicionar na Minha BVS
Imprimir
XML
PubMed Links

Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Leishmania infantum / Doenças do Cão / Reação em Cadeia da Polimerase em Tempo Real / Leishmaniose Visceral Tipo de estudo: Diagnostic_studies / Prevalence_studies / Risk_factors_studies Limite: Animals País/Região como assunto: America do sul / Brasil Idioma: En Revista: Vet Q Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Estados Unidos País de publicação: Reino Unido