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Detection of pseudocowpox virus in water buffalo (Bubalus bubalis) with vesicular disease in the state of São Paulo, Brazil, in 2016.
Laguardia-Nascimento, Mateus; de Oliveira, Ana Paula Ferreira; Fernandes, Fernanda Rodas Pires; Rivetti, Anselmo Vasconcelos; Camargos, Marcelo Fernandes; Fonseca Júnior, Antônio Augusto.
Afiliação
  • Laguardia-Nascimento M; a Laboratório de Biologia Molecular, Laboratório Nacional Agropecuário de Minhas Gerais , Pedro Leopoldo , Minas Gerais , Brazil.
  • de Oliveira AP; a Laboratório de Biologia Molecular, Laboratório Nacional Agropecuário de Minhas Gerais , Pedro Leopoldo , Minas Gerais , Brazil.
  • Fernandes FR; b Laboratório de Diagnóstico de Doenças Virais, Laboratório Nacional Agropecuário de Minhas Gerais , Pedro Leopoldo , Minas Gerais , Brazil.
  • Rivetti AV; b Laboratório de Diagnóstico de Doenças Virais, Laboratório Nacional Agropecuário de Minhas Gerais , Pedro Leopoldo , Minas Gerais , Brazil.
  • Camargos MF; b Laboratório de Diagnóstico de Doenças Virais, Laboratório Nacional Agropecuário de Minhas Gerais , Pedro Leopoldo , Minas Gerais , Brazil.
  • Fonseca Júnior AA; a Laboratório de Biologia Molecular, Laboratório Nacional Agropecuário de Minhas Gerais , Pedro Leopoldo , Minas Gerais , Brazil.
Vet Q ; 37(1): 16-22, 2017 Dec.
Article em En | MEDLINE | ID: mdl-27774853
BACKGROUND: Parapoxviruses are zoonotic viruses that infect cattle, goats and sheep; there have also been reports of infections in camels, domestic cats and seals. OBJECTIVE: The objective of this report was to describe a case of vesicular disease caused by pseudocowpox virus (PCPV) in water buffalo (Bubalus bubalis) in Brazil. ANIMALS: Sixty buffalo less than 6 months old exhibited ulcers and widespread peeling of the tongue epithelium. There were no cases of vesicular disease in pigs or horses on the same property. METHODS: Samples were analysed by PCR and sequencing. Phylogenetic analysis in MEGA 7.01 was reconstructed using major envelope protein (B2L) by the Tamura three-parameter nucleotide substitution model and the maximum likelihood and neighbor joining models, both with 1000 bootstrap replicates. The genetic distance between the groups was analysed in MEGA using the maximum composite likelihood model. The rate variation among sites was modeled using gamma distribution. RESULTS: The presence of PCPV in the buffalo herd could be demonstrated in epithelium and serum. The minimum genetic distance between the isolated PCPV strain (262-2016) and orf virus and bovine papular stomatitis virus was 6.7% and 18.4%, respectively. The maximum genetic distance calculated was 4.6% when compared with a PCPV detected in a camel. Conclusions/Clinical Importance: The peculiar position of the isolated strain in the phylogenetic trees does not necessarily indicate a different kind of PCPV that infects buffalo. More samples from cattle and buffalo in Brazil must be sequenced and compared to verify if PCPV from buffalo are genetically different from samples derived from cattle.
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Búfalos / Vírus da Pseudovaríola das Vacas / Infecções por Poxviridae Tipo de estudo: Diagnostic_studies / Incidence_studies / Prognostic_studies Limite: Animals País/Região como assunto: America do sul / Brasil Idioma: En Revista: Vet Q Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Buscar no Google
Adicionar na Minha BVS
Imprimir
XML
PubMed Links

Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Búfalos / Vírus da Pseudovaríola das Vacas / Infecções por Poxviridae Tipo de estudo: Diagnostic_studies / Incidence_studies / Prognostic_studies Limite: Animals País/Região como assunto: America do sul / Brasil Idioma: En Revista: Vet Q Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido