Identification of key genes involved in nasopharyngeal carcinoma.

Jiang, Xue; Feng, Lichun; Dai, Baoqiang; Li, Liping; Lu, Weiwei

Jiang, Xue; Feng, Lichun; Dai, Baoqiang; Li, Liping; Lu, Weiwei.

Afiliação

Jiang X; Cangzhou Central Hospital, Department of Otorhinolaryngology, Cangzhou, Hebei, China.
Feng L; Cangzhou Central Hospital, Department of Otorhinolaryngology, Cangzhou, Hebei, China.
Dai B; Cangzhou Central Hospital, Department of Otorhinolaryngology, Cangzhou, Hebei, China.
Li L; Cangzhou Central Hospital, Department of Otorhinolaryngology, Cangzhou, Hebei, China.
Lu W; Cangzhou Central Hospital, Department of Otorhinolaryngology, Cangzhou, Hebei, China. Electronic address: 13933985866@163.com.

Braz J Otorhinolaryngol ; 83(6): 670-676, 2017.

Article em En | MEDLINE | ID: mdl-27765529

RESUMO

INTRODUCTION: Nasopharyngeal carcinoma is the most common cancer originating from the nasopharynx. OBJECTIVE: To study the mechanisms of nasopharyngeal carcinoma, we analyzed GSE12452 microarray data. METHODS: GSE12452 was downloaded from the Gene Expression Omnibus database and included 31 nasopharyngeal carcinoma samples and 10 normal nasopharyngeal tissue samples. The differentially expressed genes were screened by ANOVA in the PGS package. Using the BiNGO plugin in Cytoscape and pathway enrichment analysis in the PGS package, functional and pathway enrichment analyses were performed separately to predict potential functions of the differentially expressed genes. Furthermore, Transcription factor-differentially expressed gene pairs were searched, and then the transcription factor-differentially expressed gene regulatory network was visualized using Cytoscape software. RESULTS: A total of 487 genes were screened as differentially expressed genes between the nasopharyngeal carcinoma samples and the normal nasopharyngeal tissue samples. Enrichment analysis indicated that PTGS2 was involved in the regulation of biological process and small cell lung cancer. ZIC2 and OVOL1 may function in nasopharyngeal carcinoma through targeting significantly up-regulated genes (such as PTGS2, FN1, CXCL9 and CXCL10) in the Transcription factor-differentially expressed gene regulatory network (e.g., ZIC2âPTGS2 and OVOL1âCXCL10). CONCLUSION: PTGS2, FN1, CXCL9, CXCL10, ZIC2 and OVOL1 might play roles in nasopharyngeal carcinoma.

Assuntos

Carcinoma/genética; Expressão Gênica; Neoplasias Nasofaríngeas/genética; Análise de Variância; Carcinoma/patologia; Quimiocina CXCL10/genética; Quimiocina CXCL9/genética; Análise por Conglomerados; Bases de Dados Genéticas; Regulação para Baixo; Perfilação da Expressão Gênica; Redes Reguladoras de Genes; Humanos; Análise em Microsséries; Carcinoma Nasofaríngeo; Neoplasias Nasofaríngeas/patologia; Proteínas Nucleares/genética; Fatores de Transcrição/genética; Regulação para Cima

Palavras-chave

Análise de enriquecimento; Carcinoma nasofaríngeo; Differentially expressed genes; Enrichment analysis; Genes diferencialmente expressos; Nasopharyngeal carcinoma; Rede reguladora; Regulatory network

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Carcinoma / Expressão Gênica / Neoplasias Nasofaríngeas Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Humans Idioma: En Revista: Braz J Otorhinolaryngol Assunto da revista: OTORRINOLARINGOLOGIA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: China País de publicação: Brasil

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