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Immunomodulatory/inflammatory effects of geopropolis produced by Melipona fasciculata Smith in combination with doxorubicin on THP-1 cells.
Oliveira, Lucas Pires Garcia; Conte, Fernanda Lopes; Cardoso, Eliza de Oliveira; Conti, Bruno José; Santiago, Karina Basso; Golim, Marjorie de Assis; Cruz, Maria Teresa; Sforcin, José Maurício.
Afiliação
  • Oliveira LP; Department of Microbiology and Immunology, Biosciences Institute, UNESP, Botucatu, SP, Brazil.
  • Conte FL; Department of Microbiology and Immunology, Biosciences Institute, UNESP, Botucatu, SP, Brazil.
  • Cardoso EO; Department of Microbiology and Immunology, Biosciences Institute, UNESP, Botucatu, SP, Brazil.
  • Conti BJ; Department of Microbiology and Immunology, Biosciences Institute, UNESP, Botucatu, SP, Brazil.
  • Santiago KB; Department of Microbiology and Immunology, Biosciences Institute, UNESP, Botucatu, SP, Brazil.
  • Golim MA; Botucatu Blood Center, School of Medicine, UNESP, Botucatu, SP, Brazil.
  • Cruz MT; Faculty of Pharmacy, Center for Neurosciences and Cellular Biology, University of Coimbra, Coimbra, Portugal.
  • Sforcin JM; Department of Microbiology and Immunology, Biosciences Institute, UNESP, Botucatu, SP, Brazil.
J Pharm Pharmacol ; 68(12): 1551-1558, 2016 Dec.
Article em En | MEDLINE | ID: mdl-27747861
OBJECTIVES: Geopropolis (GEO) in combination with doxorubicin (DOX) reduced HEp-2 cells viability compared to GEO and DOX alone. A possible effect of this combination on the innate immunity could take place, and its effects were analysed on THP-1 cell - a human leukaemia monocytic cell line used as a model to study monocyte activity and macrophage activity, assessing cell viability, expression of cell markers and cytokine production. METHODS: THP-1 cells were incubated with GEO, DOX and their combination. Cell viability was assessed by MTT assay, cell markers expression by flow cytometry and cytokine production by ELISA. KEY FINDINGS: GEO + DOX did not affect cell viability. GEO alone or in combination increased TLR-4 and CD80 but not HLA-DR and TLR-2 expression. GEO stimulated TNF-α production while DOX alone or in combination did not affect it. GEO alone or in combination inhibited IL-6 production. CONCLUSIONS: GEO exerted a pro-inflammatory profile by increasing TLR-4 and CD80 expression and TNF-α production, favouring the activation of the immune/inflammatory response. GEO + DOX did not affect cell viability and presented an immunomodulatory action. Lower concentrations of DOX combined to GEO could be used in cancer patients, avoiding side effects and benefiting from the biological properties of GEO.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Própole / Abelhas / Monócitos / Protocolos de Quimioterapia Combinada Antineoplásica / Doxorrubicina / Mediadores da Inflamação / Inflamação / Macrófagos Tipo de estudo: Prognostic_studies Limite: Animals / Humans Idioma: En Revista: J Pharm Pharmacol Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
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XML
PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Própole / Abelhas / Monócitos / Protocolos de Quimioterapia Combinada Antineoplásica / Doxorrubicina / Mediadores da Inflamação / Inflamação / Macrófagos Tipo de estudo: Prognostic_studies Limite: Animals / Humans Idioma: En Revista: J Pharm Pharmacol Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido