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Erythrocyte nitric oxide availability and oxidative stress following exercise.
Medeiros-Lima, Daniel Jose Matos; Mendes-Ribeiro, Antonio Claudio; Brunini, Tatiana Marlowe Cunha; Martins, Marcela Anjos; Mury, Wanda Vianna; Freire, Raul Almeira; Monteiro, Walace David; Farinatti, Paulo Tarso Veras; Matsuura, Cristiane.
Afiliação
  • Medeiros-Lima DJ; Department of Pharmacology and Psychobiology, University of the State of Rio de Janeiro, Rio de Janeiro, Brazil.
  • Mendes-Ribeiro AC; Department of Pharmacology and Psychobiology, University of the State of Rio de Janeiro, Rio de Janeiro, Brazil.
  • Brunini TM; Department of Pharmacology and Psychobiology, University of the State of Rio de Janeiro, Rio de Janeiro, Brazil.
  • Martins MA; Department of Pharmacology and Psychobiology, University of the State of Rio de Janeiro, Rio de Janeiro, Brazil.
  • Mury WV; Department of Pharmacology and Psychobiology, University of the State of Rio de Janeiro, Rio de Janeiro, Brazil.
  • Freire RA; Physical Activity and Health Promotion Laboratory, University of the State of Rio de Janeiro, Rio de Janeiro, Brazil.
  • Monteiro WD; Physical Activity and Health Promotion Laboratory, University of the State of Rio de Janeiro, Rio de Janeiro, Brazil.
  • Farinatti PT; Salgado de Oliveira University Physical Activity Sciences Graduate Program, Rio de Janeiro, Brazil.
  • Matsuura C; Physical Activity and Health Promotion Laboratory, University of the State of Rio de Janeiro, Rio de Janeiro, Brazil.
Clin Hemorheol Microcirc ; 65(3): 219-228, 2017.
Article em En | MEDLINE | ID: mdl-27716653
Growing evidence has shown that acute exercise impairs erythrocyte membrane structure and function as a consequence of increased physical and chemical stress. Erythrocyte-synthesized nitric oxide (NO) is known to modulate membrane fluidity, and its bioavailability depends on the balance between its production and scavenging by reactive oxygen species. Here, we investigated whether a maximal exercise test could affect erythrocyte NO bioavailability and oxidative stress. Twelve men (26±4 years old, V̇O2peak 44.1±4.3 mL·kg-1·min-1) performed a treadmill maximal cardiopulmonary exercise test. Blood was collected at rest and immediately after exercise for erythrocytes isolation. Maximal exercise caused an increase in erythrocytes count, haemoglobin and haematocrit levels. There was no change in L-arginine influx into erythrocytes after exercise. Yet, nitric oxide synthase activity, and thus, NO production, was increased after maximal test, as well cyclic GMP levels. In relation to biomarkers of oxidative stress, maximal test resulted in increased levels of lipid peroxidation, and diminished superoxide dismutase activity. Neither glutathione peroxidase nor catalase activity was affected by maximal test. Our findings demonstrate that the increased erythrocyte membrane rigidity caused by an acute bout of exercise may be caused, in part, by an increased lipid oxidative damage caused by ROS produced exogenously.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Exercício Físico / Estresse Oxidativo / Eritrócitos / Óxido Nítrico Limite: Adult / Humans / Male Idioma: En Revista: Clin Hemorheol Microcirc Assunto da revista: ANGIOLOGIA / HEMATOLOGIA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Exercício Físico / Estresse Oxidativo / Eritrócitos / Óxido Nítrico Limite: Adult / Humans / Male Idioma: En Revista: Clin Hemorheol Microcirc Assunto da revista: ANGIOLOGIA / HEMATOLOGIA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda