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Propolis modulates miRNAs involved in TLR-4 pathway, NF-κB activation, cytokine production and in the bactericidal activity of human dendritic cells.
Conti, Bruno J; Santiago, Karina B; Cardoso, Eliza O; Freire, Paula P; Carvalho, Robson F; Golim, Marjorie A; Sforcin, José M.
Afiliação
  • Conti BJ; Department of Microbiology and Immunology, Biosciences Institute, São Paulo State University (UNESP), São Paulo, Brazil.
  • Santiago KB; Department of Microbiology and Immunology, Biosciences Institute, São Paulo State University (UNESP), São Paulo, Brazil.
  • Cardoso EO; Department of Microbiology and Immunology, Biosciences Institute, São Paulo State University (UNESP), São Paulo, Brazil.
  • Freire PP; Department of Morphology, Biosciences Institute, São Paulo State University (UNESP), São Paulo, Brazil.
  • Carvalho RF; Department of Morphology, Biosciences Institute, São Paulo State University (UNESP), São Paulo, Brazil.
  • Golim MA; Botucatu Blood Center, School of Medicine, São Paulo State University (UNESP), São Paulo, Brazil.
  • Sforcin JM; Department of Microbiology and Immunology, Biosciences Institute, São Paulo State University (UNESP), São Paulo, Brazil.
J Pharm Pharmacol ; 68(12): 1604-1612, 2016 Dec.
Article em En | MEDLINE | ID: mdl-27714813
OBJECTIVES: Dendritic cells (DCs) are antigen-presenting cells, essential for recognition and presentation of pathogens to T cells. Propolis, a resinous material produced by bees from various plants, exhibits numerous biological properties, highlighting its immunomodulatory action. Here, we assayed the effects of propolis on the maturation and function of human DCs. METHODS: DCs were generated from human monocytes and incubated with propolis and LPS. NF-κB and cytokines production were determined by ELISA. microRNA's expression was analysed by RT-qPCR and cell markers detection by flow cytometry. Colony-forming units were obtained to assess the bactericidal activity of propolis-treated DCs. KEY FINDINGS: Propolis activated DCs in the presence of LPS, inducing NF-kB, TNF-α, IL-6 and IL-10 production. The inhibition of hsa-miR-148a and hsa-miR-148b abolished the inhibitory effects on HLA-DR and pro-inflammatory cytokines. The increased expression of hsa-miR-155 may be correlated to the increase in TLR-4 and CD86 expression, maintaining LPS-induced expression of HLA-DR and CD40. Such parameters may be involved in the increased bactericidal activity of DCs against Streptococcus mutans. CONCLUSION: Propolis modulated the maturation and function of DCs and may be useful in the initial steps of the immune response, providing a novel approach to the development of DC-based strategies and for the discovery of new immunomodulators.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Própole / Streptococcus mutans / Células Dendríticas / Citocinas / NF-kappa B / MicroRNAs / Receptor 4 Toll-Like / Anti-Inflamatórios / Antibacterianos Limite: Humans Idioma: En Revista: J Pharm Pharmacol Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
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XML
PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Própole / Streptococcus mutans / Células Dendríticas / Citocinas / NF-kappa B / MicroRNAs / Receptor 4 Toll-Like / Anti-Inflamatórios / Antibacterianos Limite: Humans Idioma: En Revista: J Pharm Pharmacol Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido